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Assay Detail

CHEMBL1648868

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Functional
Agonist activity at glucocorticoid receptor in human HepG2 cells co-transfected with GRE assessed as transactivation activity by luciferase reporter gene assay
15
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HepG2
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Glucocorticoid receptor (CHEMBL2034)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tetrahydroquinoline glucocorticoid receptor agonists: discovery of a 3-hydroxyl for improving receptor selectivity.
Roach SL, Higuchi RI, Hudson AR, Adams ME, Syka PM, Mais DE, Miner JN, Marschke KB, Zhi L.
Bioorg Med Chem Lett (2011) 21 : 168 -171
PubMed 21115247 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 15 7.40 9.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1644760 2 9.10
CHEMBL131 PREDNISOLONE 4.0 1 8.28
CHEMBL1644759 1 8.15
CHEMBL1644756 1 8.13
CHEMBL258191 1 7.70
CHEMBL1644755 1 7.02
CHEMBL1644761 1 6.98
CHEMBL1644757 1 6.67
CHEMBL1644754 1 6.63
CHEMBL1644765 1 6.43
CHEMBL1644758 1 -
CHEMBL1644764 1 -
CHEMBL1644763 1 -
CHEMBL1644762 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1644760 EC50 = 0.8 nM 9.10
CHEMBL131 PREDNISOLONE EC50 = 5.3 nM 8.28
CHEMBL1644759 EC50 = 7.1 nM 8.15
CHEMBL1644756 EC50 = 7.4 nM 8.13
CHEMBL258191 EC50 = 20.0 nM 7.70
CHEMBL1644755 EC50 = 95.0 nM 7.02
CHEMBL1644761 EC50 = 105.0 nM 6.98
CHEMBL1644757 EC50 = 215.0 nM 6.67
CHEMBL1644754 EC50 = 235.0 nM 6.63
CHEMBL1644765 EC50 = 370.0 nM 6.43
CHEMBL1644760 EC50 = 483.0 nM 6.32
CHEMBL1644758 EC50 - -
CHEMBL1644764 EC50 - -
CHEMBL1644763 EC50 - -
CHEMBL1644762 EC50 - -