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Assay Detail

CHEMBL1664585

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-stimulated nitric oxide production treated for 2 hrs before LPS challenge measured after 24 hrs by ELISA
6
Total Activities
6
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

β-carboline alkaloids from Stellaria dichotoma var. lanceolata and their anti-inflammatory activity.
Chen YF, Kuo PC, Chan HH, Kuo IJ, Lin FW, Su CR, Yang ML, Li DT, Wu TS.
J Nat Prod (2010) 73 : 1993 -1998
PubMed 21090796 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 6 4.87 5.34

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL225304 PIMAGEDINE 2.0 1 5.34
CHEMBL1651329 DICHOTOMIDE X 1 4.95
CHEMBL1651328 DICHOTOMIDE III 1 4.76
CHEMBL1651332 1 4.75
CHEMBL1651331 STELLARINES B 1 4.73
CHEMBL1651330 STELLARINES A 1 4.71

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL225304 PIMAGEDINE IC50 = 4600.0 nM 5.34
CHEMBL1651329 DICHOTOMIDE X IC50 = 11300.0 nM 4.95
CHEMBL1651328 DICHOTOMIDE III IC50 = 17300.0 nM 4.76
CHEMBL1651332 IC50 = 17900.0 nM 4.75
CHEMBL1651331 STELLARINES B IC50 = 18600.0 nM 4.73
CHEMBL1651330 STELLARINES A IC50 = 19300.0 nM 4.71