Assay Detail
Binding
CHEMBL1673551
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Activation of M5-ACh receptor-FLASH/CFP expressed in HEK293 cells assessed as FRET signal
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Cell Type | HEK293 |
| Confidence | 8 — Homologous single protein target |
| Curated By | Autocuration |
Target
Muscarinic acetylcholine receptor M5 (CHEMBL2035) ↗| Type | SINGLE PROTEIN |
| Organism | Homo sapiens |
Publication
FRET-based sensors for the human M1-, M3-, and M5-acetylcholine receptors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 4 | 5.80 | 6.10 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1184 | ACETYLCHOLINE CHLORIDE | 4.0 | 1 | 6.10 |
| CHEMBL1255785 | MUSCARINE CHLORIDE | — | 1 | 5.89 |
| CHEMBL23957 | OXOTREMORINE METHIODIDE | — | 1 | 5.64 |
| CHEMBL14 | CARBACHOL | 4.0 | 1 | 5.55 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1184 | ACETYLCHOLINE CHLORIDE | EC50 | = | 790.0 | nM | 6.10 |
| CHEMBL1255785 | MUSCARINE CHLORIDE | EC50 | = | 1300.0 | nM | 5.89 |
| CHEMBL23957 | OXOTREMORINE METHIODIDE | EC50 | = | 2300.0 | nM | 5.64 |
| CHEMBL14 | CARBACHOL | EC50 | = | 2800.0 | nM | 5.55 |