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Assay Detail

CHEMBL1743433

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Mechanism based inhibition of human cytochrome P450 3A4 measured by (14C)formaldehyde production from (N-methyl-14C)-erythromycin
4
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Expert

Target

Cytochrome P450 3A4 (CHEMBL340)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Cytochrome p450 enzymes mechanism based inhibitors: common sub-structures and reactivity.
Fontana E, Dansette PM, Poli SM.
Curr Drug Metab (2005) 6 : 413 -454
PubMed 16248836 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 4.63 4.63
Kinact 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL517449 LIMONIN 2 4.63
CHEMBL85139 RUTAECARPINE 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL517449 LIMONIN Ki = 23200.0 nM 4.63
CHEMBL85139 RUTAECARPINE Ki = 107700.0 nM -
CHEMBL517449 LIMONIN Kinact = 0.266 min-1 -
CHEMBL85139 RUTAECARPINE Kinact = 0.387 min-1 -