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Assay Detail

CHEMBL1799216

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human recombinant GABA A receptor alpha1beta2gamma2S expressed in HEK293 cells assessed as inhibition of GABA-induced current by patch clamp electrophysiology
5
Total Activities
5
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

GABA-A receptor; alpha-1/beta-2/gamma-2 (CHEMBL2095172)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Synthesis and evaluation of highly potent GABA(A) receptor antagonists based on gabazine (SR-95531).
Iqbal F, Ellwood R, Mortensen M, Smart TG, Baker JR.
Bioorg Med Chem Lett (2011) 21 : 4252 -4254
PubMed 21664131 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 5 7.70 8.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1795561 1 8.52
CHEMBL1795560 1 8.15
CHEMBL1795348 1 7.96
CHEMBL1795562 1 7.40
CHEMBL32503 GABAZINE 1 6.46

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1795561 IC50 = 3.0 nM 8.52
CHEMBL1795560 IC50 = 7.0 nM 8.15
CHEMBL1795348 IC50 = 11.0 nM 7.96
CHEMBL1795562 IC50 = 40.0 nM 7.40
CHEMBL32503 GABAZINE IC50 = 349.0 nM 6.46