Skip to main content
Free research Free research Free assay review with research home and workspace preview
Quick search ChEMBL 36
Assay Detail

CHEMBL1811510

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of TPA-induced AP-1 activity expressed in human HEK293T cells treated 1 hr before TPA challenge measured after 18 hrs by FRET based beta-lactamase reporter assay
4
Total Activities
2
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Cell Type HEK-293T
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Transcription factor Jun (CHEMBL4977)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Nothospondin, a new AP-1 inhibitory quassinoid from the Cameroonian plant Nothospondias staudtii.
Diyabalanage T, Ratnayake R, Wilson JA, Henrich CJ, Beutler JA, Colburn NH, McMahon JB, Gustafson KR.
Bioorg Med Chem Lett (2011) 21 : 4397 -4399
PubMed 21733691 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 2 6.36 6.89
Inhibition 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL458347 GLAUCARUBINONE 2 6.89
CHEMBL1807644 NOTHOSPONDIN 2 5.83

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL458347 GLAUCARUBINONE EC50 = 130.0 nM 6.89
CHEMBL1807644 NOTHOSPONDIN EC50 = 1490.0 nM 5.83
CHEMBL1807644 NOTHOSPONDIN Inhibition - % -
CHEMBL458347 GLAUCARUBINONE Inhibition - % -