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Assay Detail

CHEMBL1833709

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human beta-1 adrenergic receptor site 1 expressed in cimeterol-stimulated CHO-K1 cells assessed as CRE-SPAP level by fluorescence correlation spectroscopic analysis
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-1 adrenergic receptor (CHEMBL213)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and characterization of high-affinity 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-labeled fluorescent ligands for human β-adrenoceptors.
Baker JG, Adams LA, Salchow K, Mistry SN, Middleton RJ, Hill SJ, Kellam B.
J Med Chem (2011) 54 : 6874 -6887
PubMed 21870877 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 17 7.68 8.74

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL27 PROPRANOLOL 4.0 1 8.74
CHEMBL500 PINDOLOL 4.0 1 8.62
CHEMBL1830491 1 8.46
CHEMBL266195 ALPRENOLOL 2.0 1 8.37
CHEMBL1830618 1 8.12
CHEMBL1830485 1 8.08
CHEMBL1830486 1 7.87
CHEMBL1830619 1 7.76
CHEMBL1830489 1 7.51
CHEMBL1830621 1 7.42
CHEMBL1830492 1 7.38
CHEMBL1830617 1 7.36
CHEMBL1830493 1 7.33
CHEMBL1830487 1 7.28
CHEMBL1830488 1 7.12
CHEMBL1830490 1 6.66
CHEMBL1830620 1 6.52

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL27 PROPRANOLOL Kd = 1.82 nM 8.74
CHEMBL500 PINDOLOL Kd = 2.399 nM 8.62
CHEMBL1830491 Kd = 3.467 nM 8.46
CHEMBL266195 ALPRENOLOL Kd = 4.266 nM 8.37
CHEMBL1830618 Kd = 7.586 nM 8.12
CHEMBL1830485 Kd = 8.318 nM 8.08
CHEMBL1830486 Kd = 13.49 nM 7.87
CHEMBL1830619 Kd = 17.38 nM 7.76
CHEMBL1830489 Kd = 30.9 nM 7.51
CHEMBL1830621 Kd = 38.02 nM 7.42
CHEMBL1830492 Kd = 41.69 nM 7.38
CHEMBL1830617 Kd = 43.65 nM 7.36
CHEMBL1830493 Kd = 46.77 nM 7.33
CHEMBL1830487 Kd = 52.48 nM 7.28
CHEMBL1830488 Kd = 75.86 nM 7.12
CHEMBL1830490 Kd = 218.78 nM 6.66
CHEMBL1830620 Kd = 302.0 nM 6.52