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Assay Detail

CHEMBL1833710

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human beta-1 adrenergic receptor site 1 expressed in CGP 12177-stimulated CHO-K1 cells assessed as CRE-SPAP level by fluorescence correlation spectroscopic analysis
17
Total Activities
17
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-1 adrenergic receptor (CHEMBL213)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and characterization of high-affinity 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene-labeled fluorescent ligands for human β-adrenoceptors.
Baker JG, Adams LA, Salchow K, Mistry SN, Middleton RJ, Hill SJ, Kellam B.
J Med Chem (2011) 54 : 6874 -6887
PubMed 21870877 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Kd 17 6.85 7.38

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1830618 1 7.38
CHEMBL1830491 1 7.17
CHEMBL1830493 1 7.13
CHEMBL1830619 1 7.11
CHEMBL1830485 1 7.04
CHEMBL1830492 1 6.95
CHEMBL1830617 1 6.92
CHEMBL266195 ALPRENOLOL 2.0 1 6.75
CHEMBL1830486 1 6.74
CHEMBL27 PROPRANOLOL 4.0 1 6.70
CHEMBL1830489 1 6.62
CHEMBL1830488 1 6.45
CHEMBL1830487 1 6.10
CHEMBL1830490 1 -
CHEMBL500 PINDOLOL 4.0 1 -
CHEMBL1830620 1 -
CHEMBL1830621 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1830618 Kd = 41.69 nM 7.38
CHEMBL1830491 Kd = 67.61 nM 7.17
CHEMBL1830493 Kd = 74.13 nM 7.13
CHEMBL1830619 Kd = 77.62 nM 7.11
CHEMBL1830485 Kd = 91.2 nM 7.04
CHEMBL1830492 Kd = 112.2 nM 6.95
CHEMBL1830617 Kd = 120.23 nM 6.92
CHEMBL266195 ALPRENOLOL Kd = 177.83 nM 6.75
CHEMBL1830486 Kd = 181.97 nM 6.74
CHEMBL27 PROPRANOLOL Kd = 199.53 nM 6.70
CHEMBL1830489 Kd = 239.88 nM 6.62
CHEMBL1830488 Kd = 354.81 nM 6.45
CHEMBL1830487 Kd = 794.33 nM 6.10
CHEMBL1830490 Kd < 1000.0 nM -
CHEMBL500 PINDOLOL Kd < 10000.0 nM -
CHEMBL1830620 Kd < 1000.0 nM -
CHEMBL1830621 Kd < 1000.0 nM -