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Assay Detail

CHEMBL1918878

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of HSP90beta in human SKBR3 cells assessed as down regulation of HER2 expression levels after 24 hrs by FACS analysis
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type SK-BR-3
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Heat shock protein HSP 90-beta (CHEMBL4303)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Tricyclic series of heat shock protein 90 (Hsp90) inhibitors part I: discovery of tricyclic imidazo[4,5-c]pyridines as potent inhibitors of the Hsp90 molecular chaperone.
Vallée F, Carrez C, Pilorge F, Dupuy A, Parent A, Bertin L, Thompson F, Ferrari P, Fassy F, Lamberton A, Thomas A, Arrebola R, Guerif S, Rohaut A, Certal V, Ruxer JM, Gouyon T, Delorme C, Jouanen A, Dumas J, Grépin C, Combeau C, Goulaouic H, Dereu N, Mikol V, Mailliet P, Minoux H.
J Med Chem (2011) 54 : 7206 -7219
PubMed 21972823 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 5.93 7.52

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1917878 1 7.52
CHEMBL1917729 1 6.05
CHEMBL1917727 1 5.89
CHEMBL1917728 1 5.89
CHEMBL1917726 1 4.30
CHEMBL1917724 1 -
CHEMBL1917725 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1917878 IC50 = 30.0 nM 7.52
CHEMBL1917729 IC50 = 900.0 nM 6.05
CHEMBL1917727 IC50 = 1300.0 nM 5.89
CHEMBL1917728 IC50 = 1300.0 nM 5.89
CHEMBL1917726 IC50 = 50000.0 nM 4.30
CHEMBL1917724 IC50 > 50000.0 nM -
CHEMBL1917725 IC50 > 50000.0 nM -