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Assay Detail

CHEMBL1924274

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]NMS from recombinant human M2 receptor expressed in CHO-K1 cells after 16 hrs
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO-K1
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M2 (CHEMBL211)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The discovery of AZD9164, a novel muscarinic M3 antagonist.
Mete A, Bowers K, Chevalier E, Donald DK, Edwards H, Escott KJ, Ford R, Grime K, Millichip I, Teobald B, Russell V.
Bioorg Med Chem Lett (2011) 21 : 7440 -7446
PubMed 22047691 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 10.00 10.20

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3545181 TIOTROPIUM BROMIDE 4.0 1 10.20
CHEMBL1201335 GLYCOPYRRONIUM 4.0 1 9.80
CHEMBL551466 ACLIDINIUM BROMIDE 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3545181 TIOTROPIUM BROMIDE IC50 = 0.0631 nM 10.20
CHEMBL1201335 GLYCOPYRRONIUM IC50 = 0.1585 nM 9.80
CHEMBL551466 ACLIDINIUM BROMIDE IC50 < 0.03981 nM -