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Assay Detail

CHEMBL1937181

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at recombinant human beta2 adrenoceptor expressed in HEK293T cells assessed as increase in IBMX-induced cAMP accumulation using [3H]-cAMP after 30 mins by liquid scintillation counting in presence of 100 uM boric acid
2
Total Activities
1
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK-293T
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Beta-2 adrenergic receptor (CHEMBL210)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Cell-based and in-silico studies on the high intrinsic activity of two boron-containing salbutamol derivatives at the human β₂-adrenoceptor.
Soriano-Ursúa MA, McNaught-Flores DA, Nieto-Alamilla G, Segura-Cabrera A, Correa-Basurto J, Arias-Montaño JA, Trujillo-Ferrara JG.
Bioorg Med Chem (2012) 20 : 933 -941
PubMed 22182578 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 2 6.56 6.56

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL1002 LEVOSALBUTAMOL 4.0 2 6.56

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL1002 LEVOSALBUTAMOL EC50 = 275.0 nM 6.56
CHEMBL1002 LEVOSALBUTAMOL EC50 = 275.42 nM 6.56