Assay Detail
Binding
CHEMBL1954673
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Agonist activity at human N-His6-tagged terbium-labelled NRID-SRC3 of ERalpha ligand binding domain expressed in Escherichia coli BL21 cells after 1 hr by TR-FRET assay
8
Total Activities
4
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Cell Type | BL21 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Diarylpropionitrile (DPN) enantiomers: synthesis and evaluation of estrogen receptor β-selective ligands.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| EC50 | 4 | 8.62 | 9.25 |
| Activity | 4 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL135 | ESTRADIOL | 4.0 | 2 | 9.25 |
| CHEMBL198159 | — | — | 2 | 8.54 |
| CHEMBL334773 | DIARYLPROPIONITRILE | — | 2 | 8.47 |
| CHEMBL1950809 | — | — | 2 | 8.21 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL135 | ESTRADIOL | EC50 | = | 0.56 | nM | 9.25 |
| CHEMBL198159 | — | EC50 | = | 2.9 | nM | 8.54 |
| CHEMBL334773 | DIARYLPROPIONITRILE | EC50 | = | 3.4 | nM | 8.47 |
| CHEMBL1950809 | — | EC50 | = | 6.2 | nM | 8.21 |
| CHEMBL334773 | DIARYLPROPIONITRILE | Activity | - | — | - | |
| CHEMBL135 | ESTRADIOL | Activity | - | — | - | |
| CHEMBL198159 | — | Activity | - | — | - | |
| CHEMBL1950809 | — | Activity | - | — | - |