Assay Detail
Functional
CHEMBL2025014
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Cytotoxicity against human ECV304 cells after 72 hrs by Hoechst 33258 staining based fluorescence assay
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Functional |
| Organism | Homo sapiens |
| Cell Type | ECV-304 |
| Confidence | 1 — Organism |
| Curated By | Autocuration |
Publication
Saponins can perturb biologic membranes and reduce the surface tension of aqueous solutions: a correlation?
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 4.46 | 4.89 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL404811 | DIGITONIN | — | 1 | 4.89 |
| CHEMBL447565 | ALPHA-HEDERIN | — | 1 | 4.46 |
| CHEMBL504794 | beta-ESCIN | — | 1 | 4.28 |
| CHEMBL221542 | BETA-SITOSTEROL | — | 1 | 4.21 |
| CHEMBL505747 | GINSENOSIDE RG2 | — | 1 | - |
| CHEMBL230006 | ENOXOLONE | 2.0 | 1 | - |
| CHEMBL2024316 | GLYCYRRHIZINIC ACID AMMONIUM SALT HYDRATE | — | 1 | - |
| CHEMBL1910840 | KALOPANAXSAPONIN B | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL404811 | DIGITONIN | IC50 | = | 13000.0 | nM | 4.89 |
| CHEMBL447565 | ALPHA-HEDERIN | IC50 | = | 35000.0 | nM | 4.46 |
| CHEMBL504794 | beta-ESCIN | IC50 | = | 52000.0 | nM | 4.28 |
| CHEMBL221542 | BETA-SITOSTEROL | IC50 | = | 61000.0 | nM | 4.21 |
| CHEMBL505747 | GINSENOSIDE RG2 | IC50 | > | 127000.0 | nM | - |
| CHEMBL230006 | ENOXOLONE | IC50 | = | 101000.0 | nM | - |
| CHEMBL2024316 | GLYCYRRHIZINIC ACID AMMONIUM SALT HYDRATE | IC50 | > | 119000.0 | nM | - |
| CHEMBL1910840 | KALOPANAXSAPONIN B | IC50 | > | 164000.0 | nM | - |