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Assay Detail

CHEMBL2045513

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]N-methylscopolamine from human muscarinic M5 receptor expressed in CHO cells after 120 mins by scintillation counting
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M5 (CHEMBL2035)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

1,4-dioxane, a suitable scaffold for the development of novel M₃ muscarinic receptor antagonists.
Del Bello F, Barocelli E, Bertoni S, Bonifazi A, Camalli M, Campi G, Giannella M, Matucci R, Nesi M, Pigini M, Quaglia W, Piergentili A.
J Med Chem (2012) 55 : 1783 -1787
PubMed 22243489 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 15 6.75 9.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL376897 METHSCOPOLAMINE 4.0 1 9.68
CHEMBL2042405 1 8.77
CHEMBL2042553 1 8.74
CHEMBL2042404 1 8.36
CHEMBL2042552 1 8.27
CHEMBL1231 OXYBUTYNIN 4.0 1 7.85
CHEMBL2042554 1 7.09
CHEMBL2042406 1 6.97
CHEMBL2042407 1 5.12
CHEMBL390842 1 5.11
CHEMBL2042402 1 4.99
CHEMBL2042400 1 4.58
CHEMBL2042551 1 4.50
CHEMBL2042403 1 4.48
CHEMBL2042401 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL376897 METHSCOPOLAMINE Ki = 0.2089 nM 9.68
CHEMBL2042405 Ki = 1.698 nM 8.77
CHEMBL2042553 Ki = 1.82 nM 8.74
CHEMBL2042404 Ki = 4.365 nM 8.36
CHEMBL2042552 Ki = 5.37 nM 8.27
CHEMBL1231 OXYBUTYNIN Ki = 14.13 nM 7.85
CHEMBL2042554 Ki = 81.28 nM 7.09
CHEMBL2042406 Ki = 107.15 nM 6.97
CHEMBL2042407 Ki = 7585.78 nM 5.12
CHEMBL390842 Ki = 7762.47 nM 5.11
CHEMBL2042402 Ki = 10232.93 nM 4.99
CHEMBL2042400 Ki = 26302.68 nM 4.58
CHEMBL2042551 Ki = 31622.78 nM 4.50
CHEMBL2042403 Ki = 33113.11 nM 4.48
CHEMBL2042401 Ki > 100000.0 nM -