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Assay Detail

CHEMBL2050641

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Agonist activity at human mu opioid receptor expressed in CHO cells assessed as stimulation of [33S]GTPgammaS binding after 60 mins by scintillation counting
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL233)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis, binding affinity, and functional in vitro activity of 3-benzylaminomorphinan and 3-benzylaminomorphine ligands at opioid receptors.
Neumeyer JL, Zhang B, Zhang T, Sromek AW, Knapp BI, Cohen DJ, Bidlack JM.
J Med Chem (2012) 55 : 3878 -3890
PubMed 22439881 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 14 8.02 9.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL49269 CYCLORPHAN 1 9.10
CHEMBL2048767 1 8.96
CHEMBL301160 BUTORPHAN 1 8.80
CHEMBL2048770 1 8.44
CHEMBL2048764 1 8.44
CHEMBL2048761 1 8.42
CHEMBL2048774 1 8.19
CHEMBL568877 1 8.17
CHEMBL2048762 1 8.11
CHEMBL2048771 1 8.06
CHEMBL2048768 1 7.42
CHEMBL38874 DAMGO 1 7.26
CHEMBL2048782 1 6.68
CHEMBL2048786 1 6.26

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL49269 CYCLORPHAN EC50 = 0.8 nM 9.10
CHEMBL2048767 EC50 = 1.1 nM 8.96
CHEMBL301160 BUTORPHAN EC50 = 1.6 nM 8.80
CHEMBL2048770 EC50 = 3.6 nM 8.44
CHEMBL2048764 EC50 = 3.6 nM 8.44
CHEMBL2048761 EC50 = 3.8 nM 8.42
CHEMBL2048774 EC50 = 6.5 nM 8.19
CHEMBL568877 EC50 = 6.8 nM 8.17
CHEMBL2048762 EC50 = 7.8 nM 8.11
CHEMBL2048771 EC50 = 8.8 nM 8.06
CHEMBL2048768 EC50 = 38.0 nM 7.42
CHEMBL38874 DAMGO EC50 = 55.0 nM 7.26
CHEMBL2048782 EC50 = 210.0 nM 6.68
CHEMBL2048786 EC50 = 550.0 nM 6.26