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Assay Detail

CHEMBL2050643

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human mu opioid receptor expressed in CHO cells assessed as inhibition of DAMGO-induced [33S]GTPgammaS binding after 60 mins by scintillation counting
19
Total Activities
19
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL233)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis, binding affinity, and functional in vitro activity of 3-benzylaminomorphinan and 3-benzylaminomorphine ligands at opioid receptors.
Neumeyer JL, Zhang B, Zhang T, Sromek AW, Knapp BI, Cohen DJ, Bidlack JM.
J Med Chem (2012) 55 : 3878 -3890
PubMed 22439881 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 19 7.79 9.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2048766 1 9.68
CHEMBL49269 CYCLORPHAN 1 8.77
CHEMBL2048765 1 8.70
CHEMBL2048772 1 8.46
CHEMBL2048769 1 8.32
CHEMBL2048767 1 8.17
CHEMBL2048773 1 8.12
CHEMBL568877 1 8.00
CHEMBL301160 BUTORPHAN 1 7.70
CHEMBL2048770 1 7.12
CHEMBL2048761 1 7.10
CHEMBL2048764 1 7.03
CHEMBL2048762 1 6.82
CHEMBL2048771 1 6.80
CHEMBL2048768 1 6.09
CHEMBL38874 DAMGO 1 -
CHEMBL2048774 1 -
CHEMBL2048782 1 -
CHEMBL2048786 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2048766 IC50 = 0.21 nM 9.68
CHEMBL49269 CYCLORPHAN IC50 = 1.7 nM 8.77
CHEMBL2048765 IC50 = 2.0 nM 8.70
CHEMBL2048772 IC50 = 3.5 nM 8.46
CHEMBL2048769 IC50 = 4.8 nM 8.32
CHEMBL2048767 IC50 = 6.8 nM 8.17
CHEMBL2048773 IC50 = 7.6 nM 8.12
CHEMBL568877 IC50 = 10.0 nM 8.00
CHEMBL301160 BUTORPHAN IC50 = 20.0 nM 7.70
CHEMBL2048770 IC50 = 75.0 nM 7.12
CHEMBL2048761 IC50 = 80.0 nM 7.10
CHEMBL2048764 IC50 = 94.0 nM 7.03
CHEMBL2048762 IC50 = 150.0 nM 6.82
CHEMBL2048771 IC50 = 160.0 nM 6.80
CHEMBL2048768 IC50 = 810.0 nM 6.09
CHEMBL38874 DAMGO IC50 - -
CHEMBL2048774 IC50 - -
CHEMBL2048782 IC50 - -
CHEMBL2048786 IC50 - -