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Assay Detail

CHEMBL2051429

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Functional
Agonist activity at human mu opioid receptor expressed in CHO cells assessed as inhibition of forskolin-induced cAMP accumulation after 1 hr by HTRF assay
15
Total Activities
15
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL233)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

NOpiates: Novel Dual Action Neuronal Nitric Oxide Synthase Inhibitors with μ-Opioid Agonist Activity.
Renton P, Green B, Maddaford S, Rakhit S, Andrews JS.
ACS Med Chem Lett (2012) 3 : 227 -231
PubMed 24900459 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 15 6.51 8.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL70 MORPHINE 4.0 1 8.43
CHEMBL3215668 1 7.28
CHEMBL1400 O-DESMETHYL TRAMADOL 1 6.92
CHEMBL3217001 1 6.77
CHEMBL3216999 1 6.47
CHEMBL3215667 1 6.40
CHEMBL3216560 1 6.23
CHEMBL1201776 TAPENTADOL 4.0 1 6.17
CHEMBL3217211 1 6.03
CHEMBL1237044 TRAMADOL 4.0 1 5.89
CHEMBL3217000 1 5.82
CHEMBL3216780 1 5.66
CHEMBL3215669 1 -
CHEMBL3215885 1 -
CHEMBL3217210 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL70 MORPHINE EC50 = 3.7 nM 8.43
CHEMBL3215668 EC50 = 52.0 nM 7.28
CHEMBL1400 O-DESMETHYL TRAMADOL EC50 = 120.0 nM 6.92
CHEMBL3217001 EC50 = 170.0 nM 6.77
CHEMBL3216999 EC50 = 340.0 nM 6.47
CHEMBL3215667 EC50 = 400.0 nM 6.40
CHEMBL3216560 EC50 = 590.0 nM 6.23
CHEMBL1201776 TAPENTADOL EC50 = 670.0 nM 6.17
CHEMBL3217211 EC50 = 940.0 nM 6.03
CHEMBL1237044 TRAMADOL EC50 = 1300.0 nM 5.89
CHEMBL3217000 EC50 = 1500.0 nM 5.82
CHEMBL3216780 EC50 = 2200.0 nM 5.66
CHEMBL3215669 EC50 - -
CHEMBL3215885 EC50 - -
CHEMBL3217210 EC50 - -