Assay Detail
Binding
CHEMBL2061093
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Inhibition of recombinant phosphatase activity of Cdc25B catalytic domain expressed in Escherichia coli BL21(DE3) using OMFP as substrate by spectrofluorimetric analysis
14
Total Activities
10
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Homo sapiens |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Publication
Discovery of new inhibitors of Cdc25B dual specificity phosphatases by structure-based virtual screening.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 10 | 5.15 | 7.00 |
| Inhibition | 4 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL1481974 | — | — | 2 | 7.00 |
| CHEMBL2059274 | — | — | 1 | 5.38 |
| CHEMBL1481453 | — | — | 1 | 5.10 |
| CHEMBL1408031 | — | — | 1 | 5.00 |
| CHEMBL1414990 | — | — | 2 | 4.76 |
| CHEMBL2059074 | — | — | 1 | 4.70 |
| CHEMBL1333982 | — | — | 1 | 4.64 |
| CHEMBL1370674 | — | — | 1 | 4.62 |
| CHEMBL1270061 | — | — | 2 | - |
| CHEMBL507159 | — | — | 2 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL1481974 | — | IC50 | = | 100.0 | nM | 7.00 |
| CHEMBL2059274 | — | IC50 | = | 4200.0 | nM | 5.38 |
| CHEMBL1481453 | — | IC50 | = | 7900.0 | nM | 5.10 |
| CHEMBL1408031 | — | IC50 | = | 9900.0 | nM | 5.00 |
| CHEMBL1414990 | — | IC50 | = | 17500.0 | nM | 4.76 |
| CHEMBL2059074 | — | IC50 | = | 20100.0 | nM | 4.70 |
| CHEMBL1333982 | — | IC50 | = | 23100.0 | nM | 4.64 |
| CHEMBL1370674 | — | IC50 | = | 23800.0 | nM | 4.62 |
| CHEMBL1270061 | — | IC50 | = | 173000.0 | nM | - |
| CHEMBL507159 | — | IC50 | = | 133000.0 | nM | - |
| CHEMBL1414990 | — | Inhibition | - | % | - | |
| CHEMBL1270061 | — | Inhibition | - | % | - | |
| CHEMBL507159 | — | Inhibition | - | % | - | |
| CHEMBL1481974 | — | Inhibition | - | % | - |