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Assay Detail

CHEMBL2065510

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Inhibition of TNFalpha-induced necroptosis in FEDD-deficient human Jurkat T cells after 24 hrs by ATP-based viability assay
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type Jurkat
Confidence 1 — Organism
Curated By Autocuration

Target

Jurkat (CHEMBL397)
Type CELL-LINE
Organism Homo sapiens

Publication

Optimization of tricyclic Nec-3 necroptosis inhibitors for in vitro liver microsomal stability.
Choi S, Keys H, Staples RJ, Yuan J, Degterev A, Cuny GD.
Bioorg Med Chem Lett (2012) 22 : 5685 -5688
PubMed 22832318 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
EC50 16 6.11 7.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2064693 1 7.05
CHEMBL223735 1 6.80
CHEMBL374761 1 6.55
CHEMBL2064694 1 6.55
CHEMBL374521 1 6.54
CHEMBL2064695 1 6.48
CHEMBL222786 1 6.41
CHEMBL387398 1 6.34
CHEMBL2064698 1 6.21
CHEMBL2064696 1 6.19
CHEMBL221781 1 6.12
CHEMBL390560 1 4.92
CHEMBL2064691 1 4.82
CHEMBL2064697 1 4.57
CHEMBL2064690 1 -
CHEMBL2064692 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2064693 EC50 = 90.0 nM 7.05
CHEMBL223735 EC50 = 160.0 nM 6.80
CHEMBL374761 EC50 = 280.0 nM 6.55
CHEMBL2064694 EC50 = 280.0 nM 6.55
CHEMBL374521 EC50 = 290.0 nM 6.54
CHEMBL2064695 EC50 = 330.0 nM 6.48
CHEMBL222786 EC50 = 390.0 nM 6.41
CHEMBL387398 EC50 = 460.0 nM 6.34
CHEMBL2064698 EC50 = 610.0 nM 6.21
CHEMBL2064696 EC50 = 640.0 nM 6.19
CHEMBL221781 EC50 = 750.0 nM 6.12
CHEMBL390560 EC50 = 12000.0 nM 4.92
CHEMBL2064691 EC50 = 15000.0 nM 4.82
CHEMBL2064697 EC50 = 27000.0 nM 4.57
CHEMBL2064690 EC50 > 100000.0 nM -
CHEMBL2064692 EC50 > 100000.0 nM -