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Assay Detail

CHEMBL2072801

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant His6-tagged NAAA expressed in HEK293 cells using 10-cis-heptadecenoylethanolamide as substrate after 30 mins by UPLC/MS analysis
2
Total Activities
2
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

N-acylethanolamine-hydrolyzing acid amidase (CHEMBL4349)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

β-Lactones Inhibit N-acylethanolamine Acid Amidase by S-Acylation of the Catalytic N-Terminal Cysteine.
Armirotti A, Romeo E, Ponzano S, Mengatto L, Dionisi M, Karacsonyi C, Bertozzi F, Garau G, Tarozzo G, Reggiani A, Bandiera T, Tarzia G, Mor M, Piomelli D.
ACS Med Chem Lett (2012) 3 : 422 -426
PubMed 24900487 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 2 7.32 8.14

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2064166 1 8.14
CHEMBL2069631 1 6.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2064166 IC50 = 7.3 nM 8.14
CHEMBL2069631 IC50 = 314.0 nM 6.50