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Assay Detail

CHEMBL2077064

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
TP_TRANSPORTER: inhibition of MTX uptake in Xenopus laevis oocytes
6
Total Activities
6
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Xenopus laevis
Cell Type Oocyte
Confidence 8 — Homologous single protein target
Curated By Autocuration

Target

Solute carrier family 22 member 6 (CHEMBL1777665)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Interaction between methotrexate and nonsteroidal anti-inflammatory drugs in organic anion transporter.
Uwai Y, Saito H, Inui K.
Eur J Pharmacol (2000) 409 : 31 -36
PubMed 11099697 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Activity 3 - -
Ki 2 5.38 5.38
IC50 1 6.30 6.30

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL571 KETOPROFEN 4.0 1 6.30
CHEMBL6 INDOMETHACIN 4.0 1 5.38
CHEMBL101 PHENYLBUTAZONE 4.0 1 -
CHEMBL521 IBUPROFEN 4.0 1 -
CHEMBL23588 FLUFENAMIC ACID 2.0 1 -
CHEMBL424 SALICYLIC ACID 3.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL571 KETOPROFEN IC50 = 500.0 nM 6.30
CHEMBL6 INDOMETHACIN Ki = 4200.0 nM 5.38
CHEMBL101 PHENYLBUTAZONE Activity - -
CHEMBL521 IBUPROFEN Activity - -
CHEMBL23588 FLUFENAMIC ACID Activity - -
CHEMBL424 SALICYLIC ACID Ki = 1000000.0 nM -