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Assay Detail

CHEMBL2169535

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Displacement of [3H]mepyramine from histamine H1 receptor in Sprague-Dawley rat brain membrane after 2 hr by scintillation counting
9
Total Activities
9
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Tissue Brain
Subcellular Membrane
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H1 receptor (CHEMBL4701)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Shape-based reprofiling of FDA-approved drugs for the H₁ histamine receptor.
Vasudevan SR, Moore JB, Schymura Y, Churchill GC.
J Med Chem (2012) 55 : 7054 -7060
PubMed 22793499 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 6.04 8.97

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL908 CHLORPROTHIXENE 4.0 1 8.97
CHEMBL511 PYRILAMINE 4.0 1 8.24
CHEMBL900 ORPHENADRINE 4.0 1 6.79
CHEMBL564 PROMAZINE 4.0 1 5.90
CHEMBL86715 PROCYCLIDINE 4.0 1 5.17
CHEMBL122270 LOBELINE 2.0 1 4.95
CHEMBL597 PHENTOLAMINE 4.0 1 4.90
CHEMBL353846 ADIPHENINE 2.0 1 4.77
CHEMBL596 FENTANYL 4.0 1 4.70

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL908 CHLORPROTHIXENE IC50 = 1.072 nM 8.97
CHEMBL511 PYRILAMINE IC50 = 5.754 nM 8.24
CHEMBL900 ORPHENADRINE IC50 = 162.18 nM 6.79
CHEMBL564 PROMAZINE IC50 = 1258.93 nM 5.90
CHEMBL86715 PROCYCLIDINE IC50 = 6760.83 nM 5.17
CHEMBL122270 LOBELINE IC50 = 11220.18 nM 4.95
CHEMBL597 PHENTOLAMINE IC50 = 12589.25 nM 4.90
CHEMBL353846 ADIPHENINE IC50 = 16982.44 nM 4.77
CHEMBL596 FENTANYL IC50 = 19952.62 nM 4.70