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Assay Detail

CHEMBL2172921

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity in human THP1 cells assessed as inhibition of LPS-induced TNFalpha production after 20 hrs
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type THP-1
Confidence 1 — Organism
Curated By Autocuration

Target

THP-1 (CHEMBL614245)
Type CELL-LINE
Organism Homo sapiens

Publication

Identification, synthesis, and biological evaluation of 6-[(6R)-2-(4-fluorophenyl)-6-(hydroxymethyl)-4,5,6,7-tetrahydropyrazolo[1,5-a]pyrimidin-3-yl]-2-(2-methylphenyl)pyridazin-3(2H)-one (AS1940477), a potent p38 MAP kinase inhibitor.
Asano T, Yamazaki H, Kasahara C, Kubota H, Kontani T, Harayama Y, Ohno K, Mizuhara H, Yokomoto M, Misumi K, Kinoshita T, Ohta M, Takeuchi M.
J Med Chem (2012) 55 : 7772 -7785
PubMed 22905713 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 8.60 9.43

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2170284 1 9.43
CHEMBL2170297 1 9.34
CHEMBL2170294 AS1940477 1 9.22
CHEMBL2170292 1 9.07
CHEMBL2170295 1 9.02
CHEMBL2170290 1 9.00
CHEMBL2170296 1 8.96
CHEMBL2170288 1 8.96
CHEMBL2170293 1 8.74
CHEMBL2170287 1 8.22
CHEMBL2170291 1 8.00
CHEMBL2170286 1 7.85
CHEMBL2170285 1 7.64
CHEMBL2170289 1 6.97

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2170284 IC50 = 0.37 nM 9.43
CHEMBL2170297 IC50 = 0.46 nM 9.34
CHEMBL2170294 AS1940477 IC50 = 0.6 nM 9.22
CHEMBL2170292 IC50 = 0.85 nM 9.07
CHEMBL2170295 IC50 = 0.95 nM 9.02
CHEMBL2170290 IC50 = 1.0 nM 9.00
CHEMBL2170296 IC50 = 1.1 nM 8.96
CHEMBL2170288 IC50 = 1.1 nM 8.96
CHEMBL2170293 IC50 = 1.8 nM 8.74
CHEMBL2170287 IC50 = 6.0 nM 8.22
CHEMBL2170291 IC50 = 10.0 nM 8.00
CHEMBL2170286 IC50 = 14.0 nM 7.85
CHEMBL2170285 IC50 = 23.0 nM 7.64
CHEMBL2170289 IC50 = 106.0 nM 6.97