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Assay Detail

CHEMBL2176531

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human PDE4A-mediated [3H]CAMP hydrolysis after 30 to 60 mins by scintillation proximity assay
11
Total Activities
11
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

3',5'-cyclic-AMP phosphodiesterase 4A (CHEMBL254)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Solubility-driven optimization of phosphodiesterase-4 inhibitors leading to a clinical candidate.
Press NJ, Taylor RJ, Fullerton JD, Tranter P, McCarthy C, Keller TH, Arnold N, Beer D, Brown L, Cheung R, Christie J, Denholm A, Haberthuer S, Hatto JD, Keenan M, Mercer MK, Oakman H, Sahri H, Tuffnell AR, Tweed M, Tyler JW, Wagner T, Fozard JR, Trifilieff A.
J Med Chem (2012) 55 : 7472 -7479
PubMed 22889281 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 11 6.64 7.57

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2172376 1 7.57
CHEMBL2172373 1 6.97
CHEMBL2172374 1 6.91
CHEMBL2172375 1 6.80
CHEMBL2172372 1 6.62
CHEMBL2172370 1 6.57
CHEMBL2172369 1 6.44
CHEMBL2172367 1 6.43
CHEMBL2172366 1 6.27
CHEMBL2172368 1 6.23
CHEMBL2172371 1 6.23

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2172376 IC50 = 27.0 nM 7.57
CHEMBL2172373 IC50 = 107.0 nM 6.97
CHEMBL2172374 IC50 = 123.0 nM 6.91
CHEMBL2172375 IC50 = 160.0 nM 6.80
CHEMBL2172372 IC50 = 242.0 nM 6.62
CHEMBL2172370 IC50 = 271.0 nM 6.57
CHEMBL2172369 IC50 = 364.0 nM 6.44
CHEMBL2172367 IC50 = 371.0 nM 6.43
CHEMBL2172366 IC50 = 537.0 nM 6.27
CHEMBL2172368 IC50 = 587.0 nM 6.23
CHEMBL2172371 IC50 = 587.0 nM 6.23