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Assay Detail

CHEMBL2185189

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Time dependent inhibition of CYP3A4 in human liver microsomes
5
Total Activities
3
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Tissue Liver
Subcellular Microsome
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 3A4 (CHEMBL340)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Mechanism-based inactivation (MBI) of cytochrome P450 enzymes: structure-activity relationships and discovery strategies to mitigate drug-drug interaction risks.
Orr ST, Ripp SL, Ballard TE, Henderson JL, Scott DO, Obach RS, Sun H, Kalgutkar AS.
J Med Chem (2012) 55 : 4896 -4933
PubMed 22409598 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 2 5.06 5.20
Kinact 2 - -
IC50 1 7.82 7.82

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL623 NEFAZODONE 4.0 1 7.82
CHEMBL5416410 DASATINIB 4.0 2 5.20
CHEMBL1084546 PF-00562271 1.0 2 4.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL623 NEFAZODONE IC50 = 15.0 nM 7.82
CHEMBL5416410 DASATINIB Ki = 6300.0 nM 5.20
CHEMBL1084546 PF-00562271 Ki = 12000.0 nM 4.92
CHEMBL1084546 PF-00562271 Kinact = 0.23 /min -
CHEMBL5416410 DASATINIB Kinact = 0.034 /min -