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Assay Detail

CHEMBL2187635

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Competitive inhibition of wild-type full-length amino-terminal polyhistidine-tagged human Akt1 expressed in recombinant baculovirus system using fluorescence labeled substrate after 60 mins by fluorescence polarization assay in presence of ATP
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

RAC-alpha serine/threonine-protein kinase (CHEMBL4282)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery and preclinical pharmacology of a selective ATP-competitive Akt inhibitor (GDC-0068) for the treatment of human tumors.
Blake JF, Xu R, Bencsik JR, Xiao D, Kallan NC, Schlachter S, Mitchell IS, Spencer KL, Banka AL, Wallace EM, Gloor SL, Martinson M, Woessner RD, Vigers GP, Brandhuber BJ, Liang J, Safina BS, Li J, Zhang B, Chabot C, Do S, Lee L, Oeh J, Sampath D, Lee BB, Lin K, Liederer BM, Skelton NJ.
J Med Chem (2012) 55 : 8110 -8127
PubMed 22934575 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 8.01 8.70

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2177378 1 8.70
CHEMBL2177387 1 8.70
CHEMBL2177361 1 8.52
CHEMBL2177388 1 8.52
CHEMBL2177379 1 8.52
CHEMBL2177368 1 8.52
CHEMBL2177372 1 8.52
CHEMBL2178597 1 8.52
CHEMBL2177381 1 8.40
CHEMBL2177367 1 8.30
CHEMBL2177366 1 8.30
CHEMBL2177390 IPATASERTIB 3.0 1 8.30
CHEMBL2177374 1 8.30
CHEMBL2177363 1 8.22
CHEMBL2178598 1 8.22
CHEMBL2177385 1 8.22
CHEMBL2177377 1 8.10
CHEMBL2177369 1 8.05
CHEMBL2177364 1 8.05
CHEMBL2177375 1 7.92
CHEMBL2177382 1 7.92
CHEMBL2177371 1 7.92
CHEMBL2177362 1 7.92
CHEMBL2177370 1 7.92
CHEMBL2177365 1 7.75
CHEMBL2177373 1 7.62
CHEMBL2177380 1 7.17
CHEMBL2177389 1 7.09
CHEMBL2177376 1 6.04
CHEMBL2177383 1 5.93

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2177378 IC50 = 2.0 nM 8.70
CHEMBL2177387 IC50 = 2.0 nM 8.70
CHEMBL2177361 IC50 = 3.0 nM 8.52
CHEMBL2177388 IC50 = 3.0 nM 8.52
CHEMBL2177379 IC50 = 3.0 nM 8.52
CHEMBL2177368 IC50 = 3.0 nM 8.52
CHEMBL2177372 IC50 = 3.0 nM 8.52
CHEMBL2178597 IC50 = 3.0 nM 8.52
CHEMBL2177381 IC50 = 4.0 nM 8.40
CHEMBL2177367 IC50 = 5.0 nM 8.30
CHEMBL2177366 IC50 = 5.0 nM 8.30
CHEMBL2177390 IPATASERTIB IC50 = 5.0 nM 8.30
CHEMBL2177374 IC50 = 5.0 nM 8.30
CHEMBL2177363 IC50 = 6.0 nM 8.22
CHEMBL2177385 IC50 = 6.0 nM 8.22
CHEMBL2178598 IC50 = 6.0 nM 8.22
CHEMBL2177377 IC50 = 8.0 nM 8.10
CHEMBL2177369 IC50 = 9.0 nM 8.05
CHEMBL2177364 IC50 = 9.0 nM 8.05
CHEMBL2177362 IC50 = 12.0 nM 7.92
CHEMBL2177370 IC50 = 12.0 nM 7.92
CHEMBL2177371 IC50 = 12.0 nM 7.92
CHEMBL2177382 IC50 = 12.0 nM 7.92
CHEMBL2177375 IC50 = 12.0 nM 7.92
CHEMBL2177365 IC50 = 18.0 nM 7.75
CHEMBL2177373 IC50 = 24.0 nM 7.62
CHEMBL2177380 IC50 = 68.0 nM 7.17
CHEMBL2177389 IC50 = 81.0 nM 7.09
CHEMBL2177376 IC50 = 902.0 nM 6.04
CHEMBL2177383 IC50 = 1169.0 nM 5.93
CHEMBL2177386 IC50 > 2000.0 nM -