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Assay Detail

CHEMBL2320300

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Binding
Inhibition of human MATE2K-mediated ASP+ uptake expressed in HEK293 cells up to 500 uM after 1.5 mins by fluorescence assay
11
Total Activities
11
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Multidrug and toxin extrusion protein 2 (CHEMBL1743127)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of potent, selective multidrug and toxin extrusion transporter 1 (MATE1, SLC47A1) inhibitors through prescription drug profiling and computational modeling.
Wittwer MB, Zur AA, Khuri N, Kido Y, Kosaka A, Zhang X, Morrissey KM, Sali A, Huang Y, Giacomini KM.
J Med Chem (2013) 56 : 781 -795
PubMed 23241029 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 9 5.58 6.80
Inhibition 2 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL46 ONDANSETRON 4.0 1 6.80
CHEMBL941 IMATINIB 4.0 1 6.46
CHEMBL58 MITOXANTRONE 4.0 1 6.28
CHEMBL55 PENTAMIDINE 4.0 1 5.57
CHEMBL163 RITONAVIR 4.0 1 5.36
CHEMBL360861 NIFEKALANT 3.0 1 5.19
CHEMBL5483011 INDINAVIR 3.0 1 5.11
CHEMBL481 IRINOTECAN 4.0 1 5.10
CHEMBL1502 PANTOPRAZOLE 4.0 1 4.37
CHEMBL364713 NOSCAPINE 2.0 1 -
CHEMBL723 CARVEDILOL 4.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL46 ONDANSETRON IC50 = 160.0 nM 6.80
CHEMBL941 IMATINIB IC50 = 350.0 nM 6.46
CHEMBL58 MITOXANTRONE IC50 = 530.0 nM 6.28
CHEMBL55 PENTAMIDINE IC50 = 2700.0 nM 5.57
CHEMBL163 RITONAVIR IC50 = 4400.0 nM 5.36
CHEMBL360861 NIFEKALANT IC50 = 6500.0 nM 5.19
CHEMBL5483011 INDINAVIR IC50 = 7800.0 nM 5.11
CHEMBL481 IRINOTECAN IC50 = 7900.0 nM 5.10
CHEMBL1502 PANTOPRAZOLE IC50 = 43200.0 nM 4.37
CHEMBL364713 NOSCAPINE Inhibition - % -
CHEMBL723 CARVEDILOL Inhibition - % -