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Assay Detail

CHEMBL2329083

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat recombinant GST-fused DYRK1A expressed in Escherichia coli using KKISGRLSPIMTEQ as substrate after 30 mins by scintillation counting analysis in presence of [gamma-33P]-ATP
36
Total Activities
36
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Dual specificity tyrosine-phosphorylation-regulated kinase 1A (CHEMBL5508)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Novel Inverse Binding Mode of Indirubin Derivatives Yields Improved Selectivity for DYRK Kinases.
Myrianthopoulos V, Kritsanida M, Gaboriaud-Kolar N, Magiatis P, Ferandin Y, Durieu E, Lozach O, Cappel D, Soundararajan M, Filippakopoulos P, Sherman W, Knapp S, Meijer L, Mikros E, Skaltsounis AL.
ACS Med Chem Lett (2013) 4 : 22 -26
PubMed 23336033 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 36 6.07 6.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2321961 1 6.96
CHEMBL2321958 1 6.89
CHEMBL1233659 1 6.68
CHEMBL2321952 1 6.51
CHEMBL2321878 1 6.39
CHEMBL2321962 1 6.22
CHEMBL2321959 1 6.20
CHEMBL2321948 1 5.96
CHEMBL2321945 1 5.89
CHEMBL2321964 1 5.80
CHEMBL409450 6BIO 1 5.77
CHEMBL373834 1 5.72
CHEMBL2321968 1 5.70
CHEMBL2321965 1 5.68
CHEMBL2321974 1 5.58
CHEMBL2321963 1 5.16
CHEMBL2321967 1 -
CHEMBL375870 1 -
CHEMBL2321947 1 -
CHEMBL2321957 1 -
CHEMBL2321956 1 -
CHEMBL2321955 1 -
CHEMBL2321954 1 -
CHEMBL2321953 1 -
CHEMBL2321951 1 -
CHEMBL213454 1 -
CHEMBL2321949 1 -
CHEMBL2321960 1 -
CHEMBL2321946 1 -
CHEMBL2322019 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2321961 IC50 = 110.0 nM 6.96
CHEMBL2321958 IC50 = 130.0 nM 6.89
CHEMBL1233659 IC50 = 210.0 nM 6.68
CHEMBL2321952 IC50 = 310.0 nM 6.51
CHEMBL2321878 IC50 = 410.0 nM 6.39
CHEMBL2321962 IC50 = 600.0 nM 6.22
CHEMBL2321959 IC50 = 630.0 nM 6.20
CHEMBL2321948 IC50 = 1100.0 nM 5.96
CHEMBL2321945 IC50 = 1300.0 nM 5.89
CHEMBL2321964 IC50 = 1600.0 nM 5.80
CHEMBL409450 6BIO IC50 = 1700.0 nM 5.77
CHEMBL373834 IC50 = 1900.0 nM 5.72
CHEMBL2321968 IC50 = 2000.0 nM 5.70
CHEMBL2321965 IC50 = 2100.0 nM 5.68
CHEMBL2321974 IC50 = 2600.0 nM 5.58
CHEMBL2321963 IC50 = 7000.0 nM 5.16
CHEMBL2321969 IC50 > 10000.0 nM -
CHEMBL2321970 IC50 > 10000.0 nM -
CHEMBL2321971 IC50 > 10000.0 nM -
CHEMBL2321972 IC50 > 10000.0 nM -
CHEMBL2321973 IC50 > 10000.0 nM -
CHEMBL2321975 IC50 > 10000.0 nM -
CHEMBL2322019 IC50 > 10000.0 nM -
CHEMBL2321946 IC50 > 10000.0 nM -
CHEMBL2321960 IC50 > 10000.0 nM -
CHEMBL2321949 IC50 > 10000.0 nM -
CHEMBL213454 IC50 > 10000.0 nM -
CHEMBL2321951 IC50 > 10000.0 nM -
CHEMBL2321953 IC50 > 10000.0 nM -
CHEMBL2321954 IC50 > 10000.0 nM -
CHEMBL2321955 IC50 > 10000.0 nM -
CHEMBL2321956 IC50 > 10000.0 nM -
CHEMBL2321957 IC50 > 10000.0 nM -
CHEMBL2321947 IC50 > 10000.0 nM -
CHEMBL375870 IC50 > 10000.0 nM -
CHEMBL2321967 IC50 > 10000.0 nM -