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Assay Detail

CHEMBL2339175

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of arachidonic acid-induced COX1 activity in mouse J774 cells assessed as decrease in PGE2 levels incubated for 15 mins prior to arachidonic acid-challenge measured after 30 mins by RIA
14
Total Activities
14
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Mus musculus
Cell Type J774
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Prostaglandin G/H synthase 1 (CHEMBL2649)
Type SINGLE PROTEIN
Organism Mus musculus

Publication

Novel analgesic/anti-inflammatory agents: 1,5-diarylpyrrole nitrooxyalkyl ethers and related compounds as cyclooxygenase-2 inhibiting nitric oxide donors.
Anzini M, Di Capua A, Valenti S, Brogi S, Rovini M, Giuliani G, Cappelli A, Vomero S, Chiasserini L, Sega A, Poce G, Giorgi G, Calderone V, Martelli A, Testai L, Sautebin L, Rossi A, Pace S, Ghelardini C, Di Cesare Mannelli L, Benetti V, Giordani A, Anzellotti P, Dovizio M, Patrignani P, Biava M.
J Med Chem (2013) 56 : 3191 -3206
PubMed 23534442 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 14 5.49 5.54

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2337405 1 5.54
CHEMBL2337398 1 5.43
CHEMBL2337399 1 -
CHEMBL2337397 1 -
CHEMBL2337396 1 -
CHEMBL2337409 1 -
CHEMBL2337408 1 -
CHEMBL2337407 1 -
CHEMBL2337406 1 -
CHEMBL2337404 1 -
CHEMBL2337403 1 -
CHEMBL2337402 1 -
CHEMBL2337401 1 -
CHEMBL2337400 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2337405 IC50 = 2900.0 nM 5.54
CHEMBL2337398 IC50 = 3700.0 nM 5.43
CHEMBL2337399 IC50 > 10000.0 nM -
CHEMBL2337397 IC50 > 10000.0 nM -
CHEMBL2337396 IC50 > 10000.0 nM -
CHEMBL2337409 IC50 > 10000.0 nM -
CHEMBL2337408 IC50 > 10000.0 nM -
CHEMBL2337407 IC50 > 10000.0 nM -
CHEMBL2337406 IC50 > 10000.0 nM -
CHEMBL2337404 IC50 > 10000.0 nM -
CHEMBL2337403 IC50 > 10000.0 nM -
CHEMBL2337402 IC50 > 10000.0 nM -
CHEMBL2337401 IC50 > 10000.0 nM -
CHEMBL2337400 IC50 > 10000.0 nM -