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Assay Detail

CHEMBL2343019

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Partial agonist activity at human M1 receptor in CHO cells assessed as calcium mobilization after 60 mins by FLIPR assay
2
Total Activities
1
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Muscarinic acetylcholine receptor M1 (CHEMBL216)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of novel N-substituted oxindoles as selective m1 and m4 muscarinic acetylcholine receptors partial agonists.
Sumiyoshi T, Enomoto T, Takai K, Takahashi Y, Konishi Y, Uruno Y, Tojo K, Suwa A, Matsuda H, Nakako T, Sakai M, Kitamura A, Uematsu Y, Kiyoshi A.
ACS Med Chem Lett (2013) 4 : 244 -248
PubMed 24900656 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Intrinsic activity 1 - -
EC50 1 7.92 7.92

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2336059 2 7.92

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2336059 EC50 = 12.0 nM 7.92
CHEMBL2336059 Intrinsic activity = 60.0 % -