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Assay Detail

CHEMBL2350831

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of human recombinant His6 tagged CDK9/CyclinT1 expressed in Sf21 insect cells after 40 mins by scintillation counting analysis
37
Total Activities
36
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

CDK9/cyclin T1 (CHEMBL2111389)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

Substituted 4-(thiazol-5-yl)-2-(phenylamino)pyrimidines are highly active CDK9 inhibitors: synthesis, X-ray crystal structures, structure-activity relationship, and anticancer activities.
Shao H, Shi S, Huang S, Hole AJ, Abbas AY, Baumli S, Liu X, Lam F, Foley DW, Fischer PM, Noble M, Endicott JA, Pepper C, Wang S.
J Med Chem (2013) 56 : 640 -659
PubMed 23301767 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 36 7.93 8.70
IC50 1 7.85 7.85

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2347595 1 8.70
CHEMBL2348842 1 8.70
CHEMBL46429 1 8.70
CHEMBL2348845 1 8.52
CHEMBL2348648 1 8.52
CHEMBL2348843 1 8.52
CHEMBL2348647 1 8.40
CHEMBL2347597 ASNUCICLIB 2.0 1 8.40
CHEMBL2348649 1 8.40
CHEMBL2348638 1 8.30
CHEMBL2348642 1 8.30
CHEMBL2348844 1 8.30
CHEMBL2348650 1 8.30
CHEMBL2312183 1 8.22
CHEMBL2348644 1 8.22
CHEMBL2312182 1 8.22
CHEMBL2348651 1 8.15
CHEMBL2312185 2 8.15
CHEMBL2348646 1 8.15
CHEMBL2348641 1 8.15
CHEMBL2348847 1 8.10
CHEMBL2348645 1 8.05
CHEMBL2348637 1 7.96
CHEMBL2348846 1 7.80
CHEMBL2348652 1 7.72
CHEMBL2348643 1 7.66
CHEMBL2348848 1 7.18
CHEMBL2348639 1 7.01
CHEMBL2348653 1 6.87
CHEMBL2348841 1 6.81

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2347595 Ki = 2.0 nM 8.70
CHEMBL46429 Ki = 2.0 nM 8.70
CHEMBL2348842 Ki = 2.0 nM 8.70
CHEMBL2348648 Ki = 3.0 nM 8.52
CHEMBL2348843 Ki = 3.0 nM 8.52
CHEMBL2348845 Ki = 3.0 nM 8.52
CHEMBL2348647 Ki = 4.0 nM 8.40
CHEMBL2348649 Ki = 4.0 nM 8.40
CHEMBL2347597 ASNUCICLIB Ki = 4.0 nM 8.40
CHEMBL2348844 Ki = 5.0 nM 8.30
CHEMBL2348650 Ki = 5.0 nM 8.30
CHEMBL2348642 Ki = 5.0 nM 8.30
CHEMBL2348638 Ki = 5.0 nM 8.30
CHEMBL2312183 Ki = 6.0 nM 8.22
CHEMBL2348644 Ki = 6.0 nM 8.22
CHEMBL2312182 Ki = 6.0 nM 8.22
CHEMBL2348646 Ki = 7.0 nM 8.15
CHEMBL2348651 Ki = 7.0 nM 8.15
CHEMBL2348641 Ki = 7.0 nM 8.15
CHEMBL2312185 Ki = 7.0 nM 8.15
CHEMBL2348847 Ki = 8.0 nM 8.10
CHEMBL2348645 Ki = 9.0 nM 8.05
CHEMBL2348637 Ki = 11.0 nM 7.96
CHEMBL2312185 IC50 = 14.0 nM 7.85
CHEMBL2348846 Ki = 16.0 nM 7.80
CHEMBL2348652 Ki = 19.0 nM 7.72
CHEMBL2348643 Ki = 22.0 nM 7.66
CHEMBL2348848 Ki = 66.0 nM 7.18
CHEMBL2348639 Ki = 98.0 nM 7.01
CHEMBL2348653 Ki = 134.0 nM 6.87
CHEMBL2348841 Ki = 156.0 nM 6.81
CHEMBL2348654 Ki = 245.0 nM 6.61
CHEMBL2348840 Ki = 278.0 nM 6.56
CHEMBL2347594 Ki = 932.0 nM 6.03
CHEMBL2312193 Ki > 5000.0 nM -
CHEMBL2348640 Ki > 5000.0 nM -
CHEMBL2347596 Ki > 5000.0 nM -