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Assay Detail

CHEMBL2379310

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of acetylcholine esterase (unknown origin) using acetylthiocholine as substrate incubated for 15 mins prior to substrate addition measured after 5 mins by spectrophotometry
12
Total Activities
12
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Acetylcholinesterase (CHEMBL220)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Triazolothiadiazoles and triazolothiadiazines--biologically attractive scaffolds.
Khan I, Ibrar A, Abbas N.
Eur J Med Chem (2013) 63 : 854 -868
PubMed 23603045 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 12 5.84 7.68

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL502 DONEPEZIL 4.0 1 7.68
CHEMBL2375483 1 6.46
CHEMBL2377874 1 5.75
CHEMBL2377878 1 5.75
CHEMBL2377877 1 5.75
CHEMBL2377876 1 5.75
CHEMBL2377875 1 5.75
CHEMBL2375480 1 5.75
CHEMBL2377873 1 5.75
CHEMBL2375482 1 5.75
CHEMBL2375481 1 5.75
CHEMBL211471 NEOSTIGMINE METHYLSULFATE 4.0 1 4.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL502 DONEPEZIL IC50 = 21.0 nM 7.68
CHEMBL2375483 IC50 = 344.0 nM 6.46
CHEMBL2377874 IC50 = 1780.0 nM 5.75
CHEMBL2377878 IC50 = 1780.0 nM 5.75
CHEMBL2377877 IC50 = 1780.0 nM 5.75
CHEMBL2377876 IC50 = 1780.0 nM 5.75
CHEMBL2377875 IC50 = 1780.0 nM 5.75
CHEMBL2375480 IC50 = 1780.0 nM 5.75
CHEMBL2377873 IC50 = 1780.0 nM 5.75
CHEMBL2375482 IC50 = 1780.0 nM 5.75
CHEMBL2375481 IC50 = 1780.0 nM 5.75
CHEMBL211471 NEOSTIGMINE METHYLSULFATE IC50 = 69100.0 nM 4.16