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Assay Detail

CHEMBL2393850

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of rat VAP-1 expressed in CHO cells using [14C]-benzylamine as substrate preincubated for 30 mins prior to substrate addition measured after 1 hr by scintillation spectrometric analysis
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Rattus norvegicus
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Amine oxidase [copper-containing] 3 (CHEMBL4592)
Type SINGLE PROTEIN
Organism Rattus norvegicus

Publication

Novel 1H-imidazol-2-amine derivatives as potent and orally active vascular adhesion protein-1 (VAP-1) inhibitors for diabetic macular edema treatment.
Inoue T, Morita M, Tojo T, Nagashima A, Moritomo A, Miyake H.
Bioorg Med Chem (2013) 21 : 3873 -3881
PubMed 23664164 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 5.73 8.29

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2392121 1 8.29
CHEMBL2326864 1 7.85
CHEMBL2390968 1 7.75
CHEMBL2392120 1 6.82
CHEMBL2392118 1 6.38
CHEMBL305513 1 6.00
CHEMBL2392117 1 6.00
CHEMBL2392119 1 5.32
CHEMBL2392116 1 5.03
CHEMBL1649757 1 4.36
CHEMBL2392114 1 4.20
CHEMBL14047 PHENYLGUANIDINE 1 4.11
CHEMBL1232570 1 4.10
CHEMBL2392115 1 4.05
CHEMBL1204384 1 -
CHEMBL543123 1 -
CHEMBL187951 1 -
CHEMBL329785 1 -
CHEMBL306226 BENZIMIDAZOLE -1.0 1 -
CHEMBL3143911 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2392121 IC50 = 5.1 nM 8.29
CHEMBL2326864 IC50 = 14.0 nM 7.85
CHEMBL2390968 IC50 = 18.0 nM 7.75
CHEMBL2392120 IC50 = 150.0 nM 6.82
CHEMBL2392118 IC50 = 420.0 nM 6.38
CHEMBL305513 IC50 = 1000.0 nM 6.00
CHEMBL2392117 IC50 = 1000.0 nM 6.00
CHEMBL2392119 IC50 = 4800.0 nM 5.32
CHEMBL2392116 IC50 = 9300.0 nM 5.03
CHEMBL1649757 IC50 = 44000.0 nM 4.36
CHEMBL2392114 IC50 = 63000.0 nM 4.20
CHEMBL14047 PHENYLGUANIDINE IC50 = 78000.0 nM 4.11
CHEMBL1232570 IC50 = 80000.0 nM 4.10
CHEMBL2392115 IC50 = 90000.0 nM 4.05
CHEMBL1204384 IC50 > 100000.0 nM -
CHEMBL543123 IC50 > 100000.0 nM -
CHEMBL187951 IC50 > 100000.0 nM -
CHEMBL329785 IC50 > 100000.0 nM -
CHEMBL306226 BENZIMIDAZOLE IC50 > 100000.0 nM -
CHEMBL3143911 IC50 > 100000.0 nM -