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Assay Detail

CHEMBL2394462

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of SARS coronavirus 3C-like protease cis-cleavage activity transfected in african green monkey Vero cells using SAVLQSGFRK as substrate after 5 hrs by luciferase reporter gene assay
13
Total Activities
7
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Severe acute respiratory syndrome-related coronavirus
Cell Type Vero
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Replicase polyprotein 1ab (CHEMBL5118)
Type SINGLE PROTEIN
Organism Severe acute respiratory syndrome-related coronavirus

Publication

Dieckol, a SARS-CoV 3CL(pro) inhibitor, isolated from the edible brown algae Ecklonia cava.
Park JY, Kim JH, Kwon JM, Kwon HJ, Jeong HJ, Kim YM, Kim D, Lee WS, Ryu YB.
Bioorg Med Chem (2013) 21 : 3730 -3737
PubMed 23647823 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 4.17 4.17
Inhibition 6 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL508791 DIECKOL 2 4.17
CHEMBL473159 PHLOROGLUCINOL 3.0 1 -
CHEMBL510508 PHLOROFUCOFUROECKOL A 2 -
CHEMBL459393 FUCODIPHLOROETHOL G 2 -
CHEMBL456228 2 -
CHEMBL2391423 2-PHLOROECKOL 2 -
CHEMBL559870 DIOXINODEHYDROECKOL 2 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL508791 DIECKOL IC50 = 68100.0 nM 4.17
CHEMBL473159 PHLOROGLUCINOL IC50 - -
CHEMBL510508 PHLOROFUCOFUROECKOL A Inhibition - % -
CHEMBL508791 DIECKOL Inhibition - % -
CHEMBL459393 FUCODIPHLOROETHOL G Inhibition - % -
CHEMBL456228 Inhibition - % -
CHEMBL2391423 2-PHLOROECKOL Inhibition - % -
CHEMBL559870 DIOXINODEHYDROECKOL Inhibition - % -
CHEMBL459393 FUCODIPHLOROETHOL G IC50 = 177100.0 nM -
CHEMBL510508 PHLOROFUCOFUROECKOL A IC50 = 174600.0 nM -
CHEMBL559870 DIOXINODEHYDROECKOL IC50 = 146500.0 nM -
CHEMBL2391423 2-PHLOROECKOL IC50 = 112200.0 nM -
CHEMBL456228 IC50 = 112000.0 nM -