Assay Detail
Binding
CHEMBL2395056
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of C-terminal His6-tagged recombinant SARS coronavirus 3C-like protease trans-cleavage activity expressed in Escherichia coli BL21 (DE3) using Dabcyl-KTSAVLQSGFRKME as substrate after 60 mins by FRET assay
17
Total Activities
9
Compounds Tested
2
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Severe acute respiratory syndrome-related coronavirus |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Replicase polyprotein 1ab (CHEMBL5118) ↗| Type | SINGLE PROTEIN |
| Organism | Severe acute respiratory syndrome-related coronavirus |
Publication
Dieckol, a SARS-CoV 3CL(pro) inhibitor, isolated from the edible brown algae Ecklonia cava.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 9 | 4.89 | 5.57 |
| Inhibition | 8 | - | - |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL508791 | DIECKOL | — | 2 | 5.57 |
| CHEMBL471187 | ECKOL | — | 2 | 5.06 |
| CHEMBL2391423 | 2-PHLOROECKOL | — | 2 | 4.88 |
| CHEMBL510508 | PHLOROFUCOFUROECKOL A | — | 2 | 4.78 |
| CHEMBL459393 | FUCODIPHLOROETHOL G | — | 2 | 4.65 |
| CHEMBL456228 | — | — | 2 | 4.38 |
| CHEMBL473159 | PHLOROGLUCINOL | 3.0 | 1 | - |
| CHEMBL559870 | DIOXINODEHYDROECKOL | — | 2 | - |
| CHEMBL2391422 | TRIPHLORETHOL A | — | 2 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL508791 | DIECKOL | IC50 | = | 2700.0 | nM | 5.57 |
| CHEMBL471187 | ECKOL | IC50 | = | 8800.0 | nM | 5.06 |
| CHEMBL2391423 | 2-PHLOROECKOL | IC50 | = | 13300.0 | nM | 4.88 |
| CHEMBL510508 | PHLOROFUCOFUROECKOL A | IC50 | = | 16700.0 | nM | 4.78 |
| CHEMBL459393 | FUCODIPHLOROETHOL G | IC50 | = | 22500.0 | nM | 4.65 |
| CHEMBL456228 | — | IC50 | = | 42100.0 | nM | 4.38 |
| CHEMBL510508 | PHLOROFUCOFUROECKOL A | Inhibition | - | % | - | |
| CHEMBL508791 | DIECKOL | Inhibition | - | % | - | |
| CHEMBL459393 | FUCODIPHLOROETHOL G | Inhibition | - | % | - | |
| CHEMBL456228 | — | Inhibition | - | % | - | |
| CHEMBL2391423 | 2-PHLOROECKOL | Inhibition | - | % | - | |
| CHEMBL559870 | DIOXINODEHYDROECKOL | Inhibition | - | % | - | |
| CHEMBL471187 | ECKOL | Inhibition | - | % | - | |
| CHEMBL473159 | PHLOROGLUCINOL | IC50 | > | 200000.0 | nM | - |
| CHEMBL2391422 | TRIPHLORETHOL A | IC50 | = | 164700.0 | nM | - |
| CHEMBL559870 | DIOXINODEHYDROECKOL | IC50 | = | 158300.0 | nM | - |
| CHEMBL2391422 | TRIPHLORETHOL A | Inhibition | - | % | - |