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Assay Detail

CHEMBL2399396

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at recombinant GluN1/GluN2A receptor (unknown origin) expressed in Xenopus laevis oocytes assessed as inhibition of glycine/glutamate-induced inward current at -70mV by two-electrode voltage clamp assay
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type Oocyte
Confidence 7 — Homologous single protein target
Curated By Autocuration

Target

Glutamate NMDA receptor; GRIN1/GRIN2A (CHEMBL1907604)
Type PROTEIN COMPLEX
Organism Homo sapiens

Publication

In vitro and in vivo evaluation of polymethylene tetraamine derivatives as NMDA receptor channel blockers.
Saiki R, Yoshizawa Y, Minarini A, Milelli A, Marchetti C, Tumiatti V, Toida T, Kashiwagi K, Igarashi K.
Bioorg Med Chem Lett (2013) 23 : 3901 -3904
PubMed 23692871 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 7.48 9.02

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL807 MEMANTINE 4.0 1 9.02
CHEMBL2398522 1 8.38
CHEMBL55568 1 7.96
CHEMBL2009741 1 7.05
CHEMBL27673 METHOCTRAMINE 1 6.86
CHEMBL2398521 1 6.59
CHEMBL2398520 1 6.50

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL807 MEMANTINE IC50 = 0.95 nM 9.02
CHEMBL2398522 IC50 = 4.2 nM 8.38
CHEMBL55568 IC50 = 11.0 nM 7.96
CHEMBL2009741 IC50 = 89.3 nM 7.05
CHEMBL27673 METHOCTRAMINE IC50 = 138.0 nM 6.86
CHEMBL2398521 IC50 = 256.7 nM 6.59
CHEMBL2398520 IC50 = 315.0 nM 6.50