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Assay Detail

CHEMBL2401083

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human adenosine A2A receptor expressed in HEK293 cells assessed as inhibition of NECA-induced cAMP accumulation incubated for 10 mins prior to NECA addition
7
Total Activities
7
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type HEK293
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Adenosine receptor A2a (CHEMBL251)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and biological evaluation of metabolites of 2-n-butyl-9-methyl-8-[1,2,3]triazol-2-yl-9H-purin-6-ylamine (ST1535), a potent antagonist of the A2A adenosine receptor for the treatment of Parkinson's disease.
Piersanti G, Bartoccini F, Lucarini S, Cabri W, Stasi MA, Riccioni T, Borsini F, Tarzia G, Minetti P.
J Med Chem (2013) 56 : 5456 -5463
PubMed 23789814 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 7 6.14 6.58

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2398483 1 6.58
CHEMBL2398484 1 6.38
CHEMBL197669 ST1535 1.0 1 6.37
CHEMBL2398482 1 6.35
CHEMBL2398486 1 6.00
CHEMBL2398485 1 5.68
CHEMBL2398480 1 5.64

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2398483 IC50 = 260.0 nM 6.58
CHEMBL2398484 IC50 = 420.0 nM 6.38
CHEMBL197669 ST1535 IC50 = 430.0 nM 6.37
CHEMBL2398482 IC50 = 450.0 nM 6.35
CHEMBL2398486 IC50 = 990.0 nM 6.00
CHEMBL2398485 IC50 = 2100.0 nM 5.68
CHEMBL2398480 IC50 = 2300.0 nM 5.64