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Assay Detail

CHEMBL2427158

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR L858R/T790M double mutant phosphorylation in human NCI-H1975 cells after 2 hrs by fluorescence assay
23
Total Activities
23
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type NCI-H1975
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Structure- and reactivity-based development of covalent inhibitors of the activating and gatekeeper mutant forms of the epidermal growth factor receptor (EGFR).
Ward RA, Anderton MJ, Ashton S, Bethel PA, Box M, Butterworth S, Colclough N, Chorley CG, Chuaqui C, Cross DA, Dakin LA, Debreczeni JÉ, Eberlein C, Finlay MR, Hill GB, Grist M, Klinowska TC, Lane C, Martin S, Orme JP, Smith P, Wang F, Waring MJ.
J Med Chem (2013) 56 : 7025 -7048
PubMed 23930994 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 23 6.63 8.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2426278 1 8.25
CHEMBL2426277 1 7.72
CHEMBL2426282 1 7.66
CHEMBL1173655 AFATINIB 4.0 1 7.64
CHEMBL1229592 1 7.64
CHEMBL2426286 1 7.48
CHEMBL2110732 DACOMITINIB ANHYDROUS 4.0 1 7.38
CHEMBL2426287 1 7.28
CHEMBL2426279 1 7.17
CHEMBL2424676 1 7.15
CHEMBL2426281 1 7.09
CHEMBL2426288 1 7.02
CHEMBL2426290 1 6.66
CHEMBL2426289 1 6.60
CHEMBL2426377 1 6.54
CHEMBL2426280 1 6.11
CHEMBL2426284 1 5.68
CHEMBL2426248 1 5.64
CHEMBL2426285 1 5.55
CHEMBL939 GEFITINIB 4.0 1 5.48
CHEMBL553 ERLOTINIB 4.0 1 5.12
CHEMBL2426283 1 4.89
CHEMBL2426378 1 4.82

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2426278 IC50 = 5.6 nM 8.25
CHEMBL2426277 IC50 = 19.0 nM 7.72
CHEMBL2426282 IC50 = 22.0 nM 7.66
CHEMBL1173655 AFATINIB IC50 = 23.0 nM 7.64
CHEMBL1229592 IC50 = 23.0 nM 7.64
CHEMBL2426286 IC50 = 33.0 nM 7.48
CHEMBL2110732 DACOMITINIB ANHYDROUS IC50 = 42.0 nM 7.38
CHEMBL2426287 IC50 = 53.0 nM 7.28
CHEMBL2426279 IC50 = 68.0 nM 7.17
CHEMBL2424676 IC50 = 71.0 nM 7.15
CHEMBL2426281 IC50 = 81.0 nM 7.09
CHEMBL2426288 IC50 = 96.0 nM 7.02
CHEMBL2426290 IC50 = 220.0 nM 6.66
CHEMBL2426289 IC50 = 250.0 nM 6.60
CHEMBL2426377 IC50 = 290.0 nM 6.54
CHEMBL2426280 IC50 = 770.0 nM 6.11
CHEMBL2426284 IC50 = 2100.0 nM 5.68
CHEMBL2426248 IC50 = 2300.0 nM 5.64
CHEMBL2426285 IC50 = 2800.0 nM 5.55
CHEMBL939 GEFITINIB IC50 = 3300.0 nM 5.48
CHEMBL553 ERLOTINIB IC50 = 7600.0 nM 5.12
CHEMBL2426283 IC50 = 12900.0 nM 4.89
CHEMBL2426378 IC50 = 15200.0 nM 4.82