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Assay Detail

CHEMBL2444388

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of CYP2C9 in human liver microsomes using diclofenac as substrate after 5 to 60 mins by LC-MS/MS analysis
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Tissue Liver
Subcellular Microsome
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 2C9 (CHEMBL3397)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Discovery of GSK2656157: An Optimized PERK Inhibitor Selected for Preclinical Development.
Axten JM, Romeril SP, Shu A, Ralph J, Medina JR, Feng Y, Li WH, Grant SW, Heerding DA, Minthorn E, Mencken T, Gaul N, Goetz A, Stanley T, Hassell AM, Gampe RT, Atkins C, Kumar R.
ACS Med Chem Lett (2013) 4 : 964 -968
PubMed 24900593 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 4.89 5.35

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2171124 1 5.35
CHEMBL2441342 1 4.72
CHEMBL2441340 1 4.61
CHEMBL2441346 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2171124 IC50 = 4500.0 nM 5.35
CHEMBL2441342 IC50 = 19200.0 nM 4.72
CHEMBL2441340 IC50 = 24700.0 nM 4.61
CHEMBL2441346 IC50 > 25000.0 nM -