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Assay Detail

CHEMBL2444600

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Antagonist activity at human TRPM8 expressed in CHO cells assessed as inhibition of icilin-induced 45Ca2+ uptake incubated for 10 mins prior to icilin-challenge measured after 4 mins by liquid scintillation counting analysis
31
Total Activities
31
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Publication

Synthesis and pharmacological evaluation of novel N-aryl-3,4-dihydro-1'H-spiro[chromene-2,4'-piperidine]-1'-carboxamides as TRPM8 antagonists.
Chaudhari SS, Kadam AB, Khairatkar-Joshi N, Mukhopadhyay I, Karnik PV, Raghuram A, Rao SS, Vaiyapuri TS, Wale DP, Bhosale VM, Gudi GS, Sangana RR, Thomas A.
Bioorg Med Chem (2013) 21 : 6542 -6553
PubMed 24055075 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 31 7.01 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2443061 1 8.05
CHEMBL2443079 1 8.02
CHEMBL2443076 1 7.88
CHEMBL2443063 1 7.78
CHEMBL2443085 1 7.72
CHEMBL2443077 1 7.71
CHEMBL2443075 1 7.65
CHEMBL2443086 1 7.39
CHEMBL2443074 1 7.35
CHEMBL2443060 1 7.33
CHEMBL2443084 1 7.31
CHEMBL2443062 1 7.22
CHEMBL2443073 1 7.18
CHEMBL2443072 1 7.04
CHEMBL2443078 1 7.03
CHEMBL2443083 1 7.01
CHEMBL2443071 1 6.97
CHEMBL2443070 1 6.96
CHEMBL2443066 1 6.95
CHEMBL2443089 1 6.75
CHEMBL2440148 1 6.74
CHEMBL2443068 1 6.64
CHEMBL2443065 1 6.58
CHEMBL2443081 1 6.55
CHEMBL2443088 1 6.54
CHEMBL2443067 1 6.53
CHEMBL2443064 1 6.34
CHEMBL2443087 1 6.26
CHEMBL2443069 1 6.18
CHEMBL2443082 1 6.18

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2443061 IC50 = 8.9 nM 8.05
CHEMBL2443079 IC50 = 9.5 nM 8.02
CHEMBL2443076 IC50 = 13.3 nM 7.88
CHEMBL2443063 IC50 = 16.6 nM 7.78
CHEMBL2443085 IC50 = 18.9 nM 7.72
CHEMBL2443077 IC50 = 19.5 nM 7.71
CHEMBL2443075 IC50 = 22.5 nM 7.65
CHEMBL2443086 IC50 = 41.1 nM 7.39
CHEMBL2443074 IC50 = 44.5 nM 7.35
CHEMBL2443060 IC50 = 46.3 nM 7.33
CHEMBL2443084 IC50 = 48.5 nM 7.31
CHEMBL2443062 IC50 = 60.0 nM 7.22
CHEMBL2443073 IC50 = 65.9 nM 7.18
CHEMBL2443072 IC50 = 90.1 nM 7.04
CHEMBL2443078 IC50 = 94.0 nM 7.03
CHEMBL2443083 IC50 = 97.3 nM 7.01
CHEMBL2443071 IC50 = 107.3 nM 6.97
CHEMBL2443070 IC50 = 111.0 nM 6.96
CHEMBL2443066 IC50 = 113.1 nM 6.95
CHEMBL2443089 IC50 = 177.8 nM 6.75
CHEMBL2440148 IC50 = 182.1 nM 6.74
CHEMBL2443068 IC50 = 230.4 nM 6.64
CHEMBL2443065 IC50 = 265.8 nM 6.58
CHEMBL2443081 IC50 = 280.7 nM 6.55
CHEMBL2443088 IC50 = 289.6 nM 6.54
CHEMBL2443067 IC50 = 295.2 nM 6.53
CHEMBL2443064 IC50 = 457.2 nM 6.34
CHEMBL2443087 IC50 = 543.7 nM 6.26
CHEMBL2443069 IC50 = 656.9 nM 6.18
CHEMBL2443082 IC50 = 666.9 nM 6.18
CHEMBL2443080 IC50 = 2423.0 nM 5.62