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Assay Detail

CHEMBL2444609

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Binding affinity to wild type MOR (unknown origin) expressed in CHO cells after 15 mins by Ca2+ mobilization assay
6
Total Activities
3
Compounds Tested
2
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL233)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Binding mode characterization of 6α- and 6β-N-heterocyclic substituted naltrexamine derivatives via docking in opioid receptor crystal structures and site-directed mutagenesis studies: application of the 'message-address' concept in development of mu opioid receptor selective antagonists.
Zaidi SA, Arnatt CK, He H, Selley DE, Mosier PD, Kellogg GE, Zhang Y.
Bioorg Med Chem (2013) 21 : 6405 -6413
PubMed 24055076 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
Ki 3 8.76 8.96
IC50 3 8.44 8.64

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL522143 2 8.96
CHEMBL19019 NALTREXONE 4.0 2 8.73
CHEMBL522201 2 8.59

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL522143 Ki = 1.09 nM 8.96
CHEMBL19019 NALTREXONE Ki = 1.85 nM 8.73
CHEMBL522143 IC50 = 2.29 nM 8.64
CHEMBL522201 Ki = 2.57 nM 8.59
CHEMBL19019 NALTREXONE IC50 = 3.9 nM 8.41
CHEMBL522201 IC50 = 5.42 nM 8.27