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Assay Detail

CHEMBL2445704

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Cytotoxicity against human ECV304 cells after 72 hrs by MTT assay
10
Total Activities
10
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type ECV-304
Confidence 1 — Organism
Curated By Autocuration

Target

ECV-304 (CHEMBL614035)
Type CELL-LINE
Organism Homo sapiens

Publication

The influence of saponins on cell membrane cholesterol.
Böttger S, Melzig MF.
Bioorg Med Chem (2013) 21 : 7118 -7124
PubMed 24084294 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 10 4.67 5.10

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL2442199 1 5.10
CHEMBL2442201 1 5.05
CHEMBL2442200 1 4.89
CHEMBL447565 ALPHA-HEDERIN 1 4.46
CHEMBL504794 beta-ESCIN 1 4.28
CHEMBL221542 BETA-SITOSTEROL 1 4.21
CHEMBL2442203 1 -
CHEMBL2442202 1 -
CHEMBL1910840 KALOPANAXSAPONIN B 1 -
CHEMBL441687 GLYCYRRHIZIN 2.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL2442199 IC50 = 8000.0 nM 5.10
CHEMBL2442201 IC50 = 9000.0 nM 5.05
CHEMBL2442200 IC50 = 13000.0 nM 4.89
CHEMBL447565 ALPHA-HEDERIN IC50 = 35000.0 nM 4.46
CHEMBL504794 beta-ESCIN IC50 = 52000.0 nM 4.28
CHEMBL221542 BETA-SITOSTEROL IC50 = 61000.0 nM 4.21
CHEMBL2442203 IC50 > 100000.0 nM -
CHEMBL2442202 IC50 > 100000.0 nM -
CHEMBL1910840 KALOPANAXSAPONIN B IC50 > 164000.0 nM -
CHEMBL441687 GLYCYRRHIZIN IC50 > 119000.0 nM -