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Assay Detail

CHEMBL2446560

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Functional
Antagonist activity at human histamine H3 receptor expressed in CHO cells assessed as inhibition of forskolin/R-alpha-methylhistamine-induced cAMP accumulation after 1.5 hrs by TR-FRET immunoassay
30
Total Activities
18
Compounds Tested
3
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Histamine H3 receptor (CHEMBL264)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Synthesis and evaluation of N-alkyl-S-[3-(piperidin-1-yl)propyl]isothioureas: high affinity and human/rat species-selective histamine H(3) receptor antagonists.
Harusawa S, Sawada K, Magata T, Yoneyama H, Araki L, Usami Y, Hatano K, Yamamoto K, Yamamoto D, Yamatodani A.
Bioorg Med Chem Lett (2013) 23 : 6415 -6420
PubMed 24140447 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.32 9.10
Kd 9 8.49 10.00
pA2 3 - -

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL14690 CLOBENPROPIT 2 10.00
CHEMBL2441942 2 9.60
CHEMBL2441946 2 8.70
CHEMBL2441945 2 8.70
CHEMBL2441941 2 8.70
CHEMBL2441940 2 8.10
CHEMBL2441943 2 8.00
CHEMBL2441944 2 7.90
CHEMBL2441933 1 7.70
CHEMBL2441938 1 7.50
CHEMBL2441935 2 7.40
CHEMBL2441948 2 6.90
CHEMBL2441947 2 6.90
CHEMBL2441949 2 6.50
CHEMBL2441939 1 6.50
CHEMBL2441936 1 5.90
CHEMBL2441934 1 5.80
CHEMBL2441937 1 5.20

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL14690 CLOBENPROPIT Kd = 0.1 nM 10.00
CHEMBL2441942 Kd = 0.2512 nM 9.60
CHEMBL14690 CLOBENPROPIT IC50 = 0.7943 nM 9.10
CHEMBL2441945 IC50 = 1.995 nM 8.70
CHEMBL2441946 Kd = 1.995 nM 8.70
CHEMBL2441945 Kd = 1.995 nM 8.70
CHEMBL2441941 Kd = 1.995 nM 8.70
CHEMBL2441946 IC50 = 6.31 nM 8.20
CHEMBL2441941 IC50 = 6.31 nM 8.20
CHEMBL2441942 IC50 = 6.31 nM 8.20
CHEMBL2441940 IC50 = 7.943 nM 8.10
CHEMBL2441943 Kd = 10.0 nM 8.00
CHEMBL2441944 Kd = 12.59 nM 7.90
CHEMBL2441944 IC50 = 15.85 nM 7.80
CHEMBL2441933 IC50 = 19.95 nM 7.70
CHEMBL2441943 IC50 = 31.62 nM 7.50
CHEMBL2441938 IC50 = 31.62 nM 7.50
CHEMBL2441940 Kd = 39.81 nM 7.40
CHEMBL2441935 Kd = 39.81 nM 7.40
CHEMBL2441935 IC50 = 79.43 nM 7.10
CHEMBL2441948 IC50 = 125.89 nM 6.90
CHEMBL2441947 IC50 = 125.89 nM 6.90
CHEMBL2441949 IC50 = 316.23 nM 6.50
CHEMBL2441939 IC50 = 316.23 nM 6.50
CHEMBL2441936 IC50 = 1258.93 nM 5.90
CHEMBL2441934 IC50 = 1584.89 nM 5.80
CHEMBL2441937 IC50 = 6309.57 nM 5.20
CHEMBL2441949 pA2 - -
CHEMBL2441948 pA2 - -
CHEMBL2441947 pA2 - -