Assay Detail
Binding
CHEMBL3076134
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Inhibition of [3H]nicotine binding to alpha4 nicotinic acetylcholine receptor in Mus musculus (mouse) M10 cells co-expressing beta2 nicotinic acetylcholine receptor subunits
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Mus musculus |
| Cell Type | M10 |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Neuronal acetylcholine receptor subunit alpha-4 (CHEMBL4457) ↗| Type | SINGLE PROTEIN |
| Organism | Mus musculus |
Publication
Neonicotinoid insecticides: molecular features conferring selectivity for insect versus mammalian nicotinic receptors.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 7.83 | 10.43 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL6623 | EPIBATIDINE | — | 1 | 10.43 |
| CHEMBL72675 | — | — | 1 | 8.85 |
| CHEMBL71046 | DESCYANOTHIACLOPRID | — | 1 | 8.36 |
| CHEMBL3 | NICOTINE | 4.0 | 1 | 8.15 |
| CHEMBL309804 | DESNITROIMIDACLOPRID | — | 1 | 8.09 |
| CHEMBL2270633 | — | — | 1 | 7.10 |
| CHEMBL451432 | THIACLOPRID | — | 1 | 6.07 |
| CHEMBL406819 | IMIDACLOPRID | — | 1 | 5.58 |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL6623 | EPIBATIDINE | IC50 | = | 0.037 | nM | 10.43 |
| CHEMBL72675 | — | IC50 | = | 1.4 | nM | 8.85 |
| CHEMBL71046 | DESCYANOTHIACLOPRID | IC50 | = | 4.4 | nM | 8.36 |
| CHEMBL3 | NICOTINE | IC50 | = | 7.0 | nM | 8.15 |
| CHEMBL309804 | DESNITROIMIDACLOPRID | IC50 | = | 8.2 | nM | 8.09 |
| CHEMBL2270633 | — | IC50 | = | 80.0 | nM | 7.10 |
| CHEMBL451432 | THIACLOPRID | IC50 | = | 860.0 | nM | 6.07 |
| CHEMBL406819 | IMIDACLOPRID | IC50 | = | 2600.0 | nM | 5.58 |