Assay Detail
Binding
CHEMBL3081642
Review assay metadata, readout intent, target linkage, and publication context from the same page.
Displacement of [3HIMI from nicotinic acetylcholine receptor in Musca domestica (house fly) head membranes incubated for 60 min by liquid scintillation counting method
8
Total Activities
8
Compounds Tested
1
Activity Types
0
Assay Parameters
Assay Information
| Assay Type | Binding |
| Organism | Musca domestica |
| Tissue | Head |
| Subcellular | Membrane |
| Confidence | 9 — Direct single protein target |
| Curated By | Autocuration |
Target
Nicotinic acetylcholine receptor alpha 5 subunit (CHEMBL2366408) ↗| Type | SINGLE PROTEIN |
| Organism | Musca domestica |
Publication
Asymmetric chloronicotinyl insecticide, 1-[1-(6-chloro-3-pyridyl)ethyl]-2-nitroiminoimidazolidine: preparation, resolution and biological activities toward insects and their nerve preparations.
Activity Statistics
| Type | Count | Avg pChEMBL | Best pChEMBL |
|---|---|---|---|
| IC50 | 8 | 6.21 | 7.70 |
Compounds Tested
| Compound | Name | Phase | Activities | Best pChEMBL |
|---|---|---|---|---|
| CHEMBL406819 | IMIDACLOPRID | — | 1 | 7.70 |
| CHEMBL2272996 | (S)-METHYL-IMIDACLOPRID | — | 1 | 6.72 |
| CHEMBL2272995 | (R,S)-METHYL-IMIDACLOPRID | — | 1 | 6.68 |
| CHEMBL2272997 | (R)-METHYL-IMIDACLOPRID | — | 1 | 6.02 |
| CHEMBL298826 | (-)-EPIBATIDINE | — | 1 | 5.87 |
| CHEMBL298517 | (+)-EPIBATIDINE | — | 1 | 5.55 |
| CHEMBL3 | NICOTINE | 4.0 | 1 | 4.92 |
| CHEMBL9732 | R-NICOTINE | — | 1 | - |
Activity Data
| Compound | Name | Type | Rel. | Value | Units | pChEMBL |
|---|---|---|---|---|---|---|
| CHEMBL406819 | IMIDACLOPRID | IC50 | = | 20.0 | nM | 7.70 |
| CHEMBL2272996 | (S)-METHYL-IMIDACLOPRID | IC50 | = | 190.0 | nM | 6.72 |
| CHEMBL2272995 | (R,S)-METHYL-IMIDACLOPRID | IC50 | = | 210.0 | nM | 6.68 |
| CHEMBL2272997 | (R)-METHYL-IMIDACLOPRID | IC50 | = | 950.0 | nM | 6.02 |
| CHEMBL298826 | (-)-EPIBATIDINE | IC50 | = | 1340.0 | nM | 5.87 |
| CHEMBL298517 | (+)-EPIBATIDINE | IC50 | = | 2790.0 | nM | 5.55 |
| CHEMBL3 | NICOTINE | IC50 | = | 11900.0 | nM | 4.92 |
| CHEMBL9732 | R-NICOTINE | IC50 | = | 422000.0 | nM | - |