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Assay Detail

CHEMBL3223148

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of EGFR catalytic domain (unknown origin) assessed as reduction of poly(Glu:Tyr) substrate phosphorylation after 20 mins by ELISA
16
Total Activities
16
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Epidermal growth factor receptor (CHEMBL203)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Selective inhibition of EGFR and VEGFR2 tyrosine kinases controlled by a boronic acid substituent on 4-anilinoquinazolines
Nakamura H, Horikoshi R, Usui T, Ban HS
Medchemcomm (2010) 1 : 282 -286
· DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 16 6.37 7.33

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL553 ERLOTINIB 4.0 1 7.33
CHEMBL3219507 1 6.34
CHEMBL3219502 1 6.31
CHEMBL3219508 1 6.29
CHEMBL3219503 1 6.28
CHEMBL3219505 1 6.24
CHEMBL3219504 1 6.23
CHEMBL3218424 1 6.19
CHEMBL3219506 1 6.10
CHEMBL3219509 1 -
CHEMBL3217774 1 -
CHEMBL3219510 1 -
CHEMBL3219511 1 -
CHEMBL3219512 1 -
CHEMBL3219513 1 -
CHEMBL153843 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL553 ERLOTINIB IC50 = 47.0 nM 7.33
CHEMBL3219507 IC50 = 460.0 nM 6.34
CHEMBL3219502 IC50 = 490.0 nM 6.31
CHEMBL3219508 IC50 = 510.0 nM 6.29
CHEMBL3219503 IC50 = 520.0 nM 6.28
CHEMBL3219505 IC50 = 570.0 nM 6.24
CHEMBL3219504 IC50 = 590.0 nM 6.23
CHEMBL3218424 IC50 = 650.0 nM 6.19
CHEMBL3219506 IC50 = 800.0 nM 6.10
CHEMBL3219509 IC50 > 1000.0 nM -
CHEMBL3217774 IC50 > 1000.0 nM -
CHEMBL3219510 IC50 > 1000.0 nM -
CHEMBL3219511 IC50 > 1000.0 nM -
CHEMBL3219512 IC50 > 1000.0 nM -
CHEMBL3219513 IC50 > 1000.0 nM -
CHEMBL153843 IC50 > 1000.0 nM -