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Assay Detail

CHEMBL3227490

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antagonist activity at human mu opioid receptor expressed in Gqi5 transfected CHO cells assessed as inhibition of DAMGO-stimulated Ca2+ influx preincubated for 15 mins followed by DAMGO challenge
3
Total Activities
3
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Homo sapiens
Cell Type CHO
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Mu-type opioid receptor (CHEMBL233)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

A Bivalent Ligand Targeting the Putative Mu Opioid Receptor and Chemokine Receptor CCR5 Heterodimers: Binding Affinity versus Functional Activities.
Yuan Y, Arnatt CK, El-Hage N, Dever SM, Jacob JC, Selley DE, Hauser KF, Zhang Y.
Medchemcomm (2013) 4 : 847 -851
PubMed 23682308 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 3 7.62 8.05

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL19019 NALTREXONE 4.0 1 8.05
CHEMBL3219778 1 7.42
CHEMBL3219777 1 7.40

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL19019 NALTREXONE IC50 = 8.9 nM 8.05
CHEMBL3219778 IC50 = 37.8 nM 7.42
CHEMBL3219777 IC50 = 40.0 nM 7.40