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Assay Detail

CHEMBL3268199

Review assay metadata, readout intent, target linkage, and publication context from the same page.

ADMET
Inhibition of CYP3A4 in human liver microsomes using midazolam as substrate measured after 30 mins preincubation by LC-MS/MS analysis in presence of NADPH
4
Total Activities
4
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type ADMET
Organism Homo sapiens
Tissue Liver
Subcellular Microsomes
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Cytochrome P450 3A4 (CHEMBL340)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

Optimizing the Physicochemical Properties of Raf/MEK Inhibitors by Nitrogen Scanning.
Aoki T, Hyohdoh I, Furuichi N, Ozawa S, Watanabe F, Matsushita M, Sakaitani M, Morikami K, Takanashi K, Harada N, Tomii Y, Shiraki K, Furumoto K, Tabo M, Yoshinari K, Ori K, Aoki Y, Shimma N, Iikura H.
ACS Med Chem Lett (2014) 5 : 309 -314
PubMed 24900832 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 4 5.11 5.25

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3092189 1 5.25
CHEMBL3092195 1 4.96
CHEMBL3264002 AVUTOMETINIB 3.0 1 -
CHEMBL3263996 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3092189 IC50 = 5600.0 nM 5.25
CHEMBL3092195 IC50 = 11000.0 nM 4.96
CHEMBL3264002 AVUTOMETINIB IC50 > 100000.0 nM -
CHEMBL3263996 IC50 > 100000.0 nM -