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Assay Detail

CHEMBL3299341

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Binding
Inhibition of VEGFR2 in human U251 cells by phosphotyrosine ELISA assay
18
Total Activities
18
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Binding
Organism Homo sapiens
Cell Type U-251
Confidence 9 — Direct single protein target
Curated By Autocuration

Target

Vascular endothelial growth factor receptor 2 (CHEMBL279)
Type SINGLE PROTEIN
Organism Homo sapiens

Publication

The design and discovery of water soluble 4-substituted-2,6-dimethylfuro[2,3-d]pyrimidines as multitargeted receptor tyrosine kinase inhibitors and microtubule targeting antitumor agents.
Zhang X, Raghavan S, Ihnat M, Thorpe JE, Disch BC, Bastian A, Bailey-Downs LC, Dybdal-Hargreaves NF, Rohena CC, Hamel E, Mooberry SL, Gangjee A.
Bioorg Med Chem (2014) 22 : 3753 -3772
PubMed 24890652 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 18 7.47 8.07

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL3297900 1 8.07
CHEMBL3297899 1 7.97
CHEMBL3297999 1 7.81
CHEMBL3297898 1 7.72
CHEMBL3298000 1 7.70
CHEMBL3297891 1 7.49
CHEMBL3297998 1 7.46
CHEMBL3297897 1 7.37
CHEMBL3297816 1 7.30
CHEMBL3297894 1 7.24
CHEMBL3297893 1 7.18
CHEMBL3297895 1 7.15
CHEMBL3297892 1 7.12
CHEMBL3297896 1 6.99
CHEMBL535 SUNITINIB 4.0 1 -
CHEMBL53463 DOXORUBICIN 4.0 1 -
CHEMBL553 ERLOTINIB 4.0 1 -
CHEMBL276711 SEMAXANIB 3.0 1 -

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL3297900 IC50 = 8.5 nM 8.07
CHEMBL3297899 IC50 = 10.6 nM 7.97
CHEMBL3297999 IC50 = 15.6 nM 7.81
CHEMBL3297898 IC50 = 19.1 nM 7.72
CHEMBL3298000 IC50 = 20.2 nM 7.70
CHEMBL3297891 IC50 = 32.2 nM 7.49
CHEMBL3297998 IC50 = 34.4 nM 7.46
CHEMBL3297897 IC50 = 43.1 nM 7.37
CHEMBL3297816 IC50 = 50.3 nM 7.30
CHEMBL3297894 IC50 = 58.2 nM 7.24
CHEMBL3297893 IC50 = 66.2 nM 7.18
CHEMBL3297895 IC50 = 70.2 nM 7.15
CHEMBL3297892 IC50 = 76.3 nM 7.12
CHEMBL3297896 IC50 = 102.1 nM 6.99
CHEMBL535 SUNITINIB IC50 = 18.9 nM -
CHEMBL53463 DOXORUBICIN IC50 - -
CHEMBL553 ERLOTINIB IC50 = 124.7 nM -
CHEMBL276711 SEMAXANIB IC50 = 12.9 nM -