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Assay Detail

CHEMBL3370016

Review assay metadata, readout intent, target linkage, and publication context from the same page.

Functional
Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 1 hr prior to LPS challenge measured after 24 hrs by Griess method
20
Total Activities
20
Compounds Tested
1
Activity Types
0
Assay Parameters

Assay Information

Assay Type Functional
Organism Mus musculus
Cell Type RAW264.7
Confidence 1 — Organism
Curated By Autocuration

Target

RAW264.7 (CHEMBL612557)
Type CELL-LINE
Organism Mus musculus

Publication

Synthesis of new heterocyclic lupeol derivatives as nitric oxide and pro-inflammatory cytokine inhibitors.
Bhandari P, Patel NK, Bhutani KK.
Bioorg Med Chem Lett (2014) 24 : 3596 -3599
PubMed 24909081 · DOI

Activity Statistics

Type Count Avg pChEMBL Best pChEMBL
IC50 20 4.47 4.96

Compounds Tested

Compound Name Phase Activities Best pChEMBL
CHEMBL140 CURCUMIN 3.0 1 4.96
CHEMBL3310695 1 4.65
CHEMBL3310694 1 4.62
CHEMBL3310690 1 4.59
CHEMBL3310689 1 4.56
CHEMBL3310691 1 4.51
CHEMBL3310688 1 4.50
CHEMBL3310696 1 4.49
CHEMBL3310693 1 4.45
CHEMBL3310687 1 4.44
CHEMBL453802 LUPENYL ACETATE 1 4.43
CHEMBL289191 LUPEOL 1 4.43
CHEMBL486393 LUPENONE 1 4.42
CHEMBL3310426 1 4.41
CHEMBL211376 1 4.41
CHEMBL3310692 1 4.38
CHEMBL2042333 1 4.37
CHEMBL2042330 1 4.34
CHEMBL3310425 1 4.31
CHEMBL7890 L-NAME 2.0 1 4.16

Activity Data

Compound Name Type Rel. Value Units pChEMBL
CHEMBL140 CURCUMIN IC50 = 11020.0 nM 4.96
CHEMBL3310695 IC50 = 22600.0 nM 4.65
CHEMBL3310694 IC50 = 23900.0 nM 4.62
CHEMBL3310690 IC50 = 25900.0 nM 4.59
CHEMBL3310689 IC50 = 27800.0 nM 4.56
CHEMBL3310691 IC50 = 30600.0 nM 4.51
CHEMBL3310688 IC50 = 31700.0 nM 4.50
CHEMBL3310696 IC50 = 32400.0 nM 4.49
CHEMBL3310693 IC50 = 35600.0 nM 4.45
CHEMBL3310687 IC50 = 36200.0 nM 4.44
CHEMBL453802 LUPENYL ACETATE IC50 = 36700.0 nM 4.43
CHEMBL289191 LUPEOL IC50 = 37300.0 nM 4.43
CHEMBL486393 LUPENONE IC50 = 38500.0 nM 4.42
CHEMBL3310426 IC50 = 39200.0 nM 4.41
CHEMBL211376 IC50 = 39200.0 nM 4.41
CHEMBL3310692 IC50 = 41700.0 nM 4.38
CHEMBL2042333 IC50 = 42900.0 nM 4.37
CHEMBL2042330 IC50 = 46000.0 nM 4.34
CHEMBL3310425 IC50 = 48700.0 nM 4.31
CHEMBL7890 L-NAME IC50 = 69210.0 nM 4.16